商品编号


K1201



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E3 Ubiquitin Ligases



Size

250 ?g


商品信息

The carboxyl terminus of Hsc70-interacting protein (CHIP) is an E3 ubiquitin ligase. CHIP contains two domains, one is its TPR domain on the amino terminus and the other is its U-box domain on the carboxyl terminus. The TPR domain recognizes the chaperones, Hsp70 and Hsp90. While the U-box domain carries out its ubiquitin ligase activity. CHIP often ubiquitinates misfolded proteins bound on the chaperones.

Additional Information






Product Name:

6xHis-CHIP



Also Known As:

STUB1; UBOX1; HSPABP2; NY-CO-7; SDCCAG7



Catalog No.:

K1201



Size:

250 ?g



Molecular Weight:

34.9 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~95% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_005861



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-CHIP







Shipping Method:

Dry ice shipping



References:

1. Ballinger CA,
et al
. (1999) Mol Cell Biol 19(6), 4535 – 4545.
2. Murata S,
et al
. (2003) Int J Biochem Cell Biol 35(5), 572 – 578.




Details

The carboxyl terminus of Hsc70-interacting protein (CHIP) is an E3 ubiquitin ligase. CHIP contains two domains, one is its TPR domain on the amino terminus and the other is its U-box domain on the carboxyl terminus. The TPR domain recognizes the chaperones, Hsp70 and Hsp90. While the U-box domain carries out its ubiquitin ligase activity. CHIP often ubiquitinates misfolded proteins bound on the chaperones.

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified 6xHis-CHIP