商品编号


K1400



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E3 Ubiquitin Ligases



Size

50 ?g


商品信息

ITCH, also called atrophin-1 interacting protein 4 (AIP4), is a HECT-type E3 ubiquitin ligase. It contains an N-terminal Ca<>-dependent phospholipid-binding C2 domain, four WW domains, and a C-terminal HECT domain. Mice deficient in ITCH protein display many autoimmune-like disease characteristics such as lymphoproliferation in the spleen and lymph nodes. More than a dozen of substrates of ITCH have been identified including p63, p73 and Notch1. The ligase may be respons
IBL
e for ubiquitinating proteins with K29-, K48-, and/or K63-linked ubiquitin chains.

Additional Information






Product Name:

ITCH



Also Known As:

AIF4; AIP4; NAPP1; dJ468O1.1



Catalog No.:

K1400



Size:

50 ?g



Molecular Weight:

102.8 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~90% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet
，
MSD
S



NCBI RefSeq:

NM_001257137



Image(s):

(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified ITCH







Shipping Method:

Dry ice shipping



References:

1. Perry WL,
et al
. (1998) Nat Genet 18, 143 – 146.
2. Schwarz SE,
et al
. (1998) J Biol Chem 273, 12148 – 12154. 3. Melino G,
et al
. (2008) Cell Death Differ 15(7), 1103 – 1112.




Details

ITCH, also called atrophin-1 interacting protein 4 (AIP4), is a HECT-type E3 ubiquitin ligase. It contains an N-terminal Ca<>-dependent phospholipid-binding C2 domain, four WW domains, and a C-terminal HECT domain. Mice deficient in ITCH protein display many autoimmune-like disease characteristics such as lymphoproliferation in the spleen and lymph nodes. More than a dozen of substrates of ITCH have been identified including p63, p73 and Notch1. The ligase may be respons
IBL
e for ubiquitinating proteins with K29-, K48-, and/or K63-linked ubiquitin chains.<>

Images:
(Click image to enlarge)








Coomassie-stained SDS-PAGE
Lane 1: Molecular weight
Marker
s
Lane 2: 5 ?g purified ITCH