商品编号


K1411



品牌


UBPBio



公司


Ubiquitin-Proteasome Biotechnologies, LLC



公司分类


E3 Ubiquitin Ligases



Size

50 ?g

商品信息

UbE3A (also called E6AP) is a 100 kDa HECT (homologous to E6AP carboxyl terminus) domain ubiquitin ligase containing two E2 binding sites for UbcH7 and UbcH8. UbE3A was found to bind to the human papillomavirus (HPV) oncoprotein E6 and mediate ubiquitination of p53. A frameshift mutation in the UbE3A gene leads to loss of function and causes Angelman Syndrome.

Additional Information






Product Name:

UbE3A (E6AP)



Also Known As:

AS; ANCR; E6-AP; E6AP; HPVE6A; EPVE6AP



Catalog No.:

K1411



Size:

50 ?g



Molecular Weight:

100.7 kDa



Species:

Human



Source:

Bacterial recombinant



Stock:

20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol



Concentration:

See tube label



Quality Assurance:

~90% by SDS-PAGE, see datasheet for gel image



Storage:

Store at -80°C; avoid multiple freeze-thaw cycles



PDF Data Sheet:

PDF Datasheet



NCBI RefSeq:

NM_000462.3



Image(s):

Please click product PDF datasheet above to view a representative SDS-PAGE of UbE3A.



Shipping Method:

Dry ice shipping



References:

1. Scheffner M,
et al
. (1990) Cell 63, 1129?1136.
2. Scheffner M,
et al
. (1993) Cell 75, 495?505. 3. Abaied L,
et al
. (2009) Am J Med Genet Part A 152A, 141?146.
4. Ronchi VP,
et al
. (2013) JBC 288, 10349?10360.




Details

UbE3A (also called E6AP) is a 100 kDa HECT (homologous to E6AP carboxyl terminus) domain ubiquitin ligase containing two E2 binding sites for UbcH7 and UbcH8. UbE3A was found to bind to the human papillomavirus (HPV) oncoprotein E6 and mediate ubiquitination of p53. A frameshift mutation in the UbE3A gene leads to loss of function and causes Angelman Syndrome. This product is made from GST-UbE3A (catalog # K1410) by removal of the N-terminal GST tag.