商品编号


NP-101-1001



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
The TruSeq Nano DNA for NeoPrep offers a simple, all-inclusive prep solution, ideal for any whole-genome sequencing application. Run the same proven TruSeq Nano DNA biochemistry on the fully integrated digital microfluidics NeoPrep Library Prep System.


Simplified and seamless workflow solution – Includes onboard quantification and normalization to deliver sequencing-ready libraries

Exceptional performance and reproducibility – Generates libraries with TruSeq Nano DNA coverage and quality, with minimal hands-on time and reduced user vari
ABI
lity

Low input requirement – Requires as little as 25 ng genomic DNA to prepare high-quality, sequencing-ready libraries


Reduce Library Bias and Coverage Gaps

TruSeq Nano DNA library prep reduces the number and average size of typical PCR-induced gaps in coverage. The enhanced workflow reduces library bias and improves coverage uniformity across the genome. It also provides excellent coverage of tr
ADI
tionally challenging genomic content, including GC-rich regions, promoters, and repetitive regions. This enables you to access more information from each sequencing run.


NeoPrep System enhances TruSeq Nano DNA performance

The NeoPrep System enhances TruSeq Nano DNA performance by providing high-quality reproduc
IBL
e results, even with low input amounts of DNA. Digital microfluidics technology precisely manipulates droplets that perform the library prep workflow, including quantification and normalization within the tightly controlled environment of the NeoPrep library card.

A simple, intuitive workflow delivers 16 sequencing-ready libraries, eliminating almost all manual steps, and reducing hands-on time from ~4 hours to just 30 minutes. In addition, digital microfluidics requires less DNA input, enabling excellent performance from 25-75 ng of genomic DNA. Successful libraries have been demonstrated with DNA inputs ranging from 1–100 ng, up to 10-fold lower than required by manual protocols.
Specifications:
Assay Time
1 day
Hands-On Time
4 hours
Input Quantity
50 ng RNA,50 ng high-quality total RNA,≥ 200 ng FFPE total RNA; Recommended quantity may vary with expression level, target plexity, and sample quality
Content Specifications
Choose from 400,000+ pre-designed targeted RNA-Seq assays. Or add content to a fixed panel or previously designed custom panel.
Multiplexing
Up to 384 samples per sequencing run
Mechanism of Action
Amplification
Method
Shotgun Sequencing,Whole-Genome Sequencing,Genotyping by Sequencing
Variant Class
Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels),Copy Number Variants (CNVs)
Species Category
Mammalian,Mouse,Human,Other,Rat,Plant
System Compatibility
NextSeq 550,HiSeq 3000,HiSeq X Five,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 2000,MiSeq,HiSeq X Ten,NeoPrep,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000
Specialized Sample Types
Low Input
Technology
Sequencing
Automation Cap
ABI
lity
NeoPrep Digital Microfluidics