商品编号


RS-301-2002



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
TruSeq RNA Access kits convert total RNA into template molecules of known strand origin, followed by sequence-specific capture of coding RNA. This provides a low-cost solution for analyzing human RNA isolated from FFPE (formalin-fixed, paraffin-embedded) tissues and other low-quality samples.


Afford
ABI
lity and focus
- isolating human transcriptome coding regions maximizes discovery power at a fraction of the sequencing depth

High-quality data from difficult samples
- optimized for sequencing RNA from degraded samples, including FFPE tissues

Samples with limited starting material
- greatly reduced sample input requirements (as little as 10 ng total RNA from fresh or frozen samples or 20 ng total RNA from degraded samples) while maintaining high sensitivity



TruSeq RNA Access Kit generates RNA-Seq libraries from degraded samples that focus on the RNA coding regions. Isolating these high-value content regions maximizes discovery power, while requiring only a fraction of the read depth of total RNA sequencing. The results are low input requirements, high sample throughput, and cost-effective transcriptome analysis.

Find an up-to-date list of high-throughput automation vendors with robotic systems compat
IBL
e with this library preparation kit
Specifications:
Assay Time
~2 Days
Hands-on Time
~11 hours
Input Quantity
10 ng total RNA from fresh/frozen samples, or 20 ng total RNA from FFPE samples
Content Specifications
Captures the coding transcriptome/RNA exome
Mechanism of Action
Biotinylated capture probes that target coding RNA. Does not require RNA with poly-A tails.
Multiplexing
Up to 24 unique indexes and 4-plex pre-enrichment pooling
System Compatibility
NovaSeq 5000,NextSeq 550,HiSeq 3000,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class
Single Nucleotide Polymorphisms (SNPs),Novel Transcripts,Transcript Variants
Specialized Sample Types
FFPE,Low Input
Technology
Sequencing
Method
mRNA Sequencing,Exome Sequencing
Species Category
Human
Automation Cap
ABI
lity
Liquid Handling Robots