商品编号


RS-122-2001



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
Generate mRNA-focused sequencing libraries from total RNA, with enhanced multiplex cap
ABI
lity and a simplified workflow with master-mixed reagents.

Kits feature 24 unique indexes, delivering enhanced multiplex performance for processing large numbers of samples (including up to 384 RNA-Seq samples on a single HiSeq 2500 System run).


Master-mixed reagents eliminate the majority of pipetting steps and reduce the amount of clean-up, as compared to previous methods, minimizing hands-on time. This results in economical, high-throughput RNA sequencing studies achieved with the easiest-to-use sample preparation workflow offered by any NGS platform.

Find an up-to-date list of high-throughput automation vendors with robotic systems compat
IBL
e with this library preparation kit
Specifications:
Assay Time
~10.5 hours
Hands-On Time
~4.5 hours
Input Quantity
0.1 - 1 ug total RNA or 10 - 400 ng previously isolated mRNA (from species with polyA tails)
Content Specifications
Captures the coding transcriptome (without strand information)
Mechanism of Action
Oligo-dT beads capture polyA tails
Multiplexing
Up to 24-plex per lane
System Compatibility
Genome Analyzer IIx,HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,HiSeq 4000
Species Category
Other,Mammalian,Bovine,Mouse,Human,Rat
Variant Class
Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Technology
Sequencing
Method
mRNA Sequencing
Automation Cap
ABI
lity
Liquid Handling Robots