商品编号


RPYSC12116



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:

Study active mRNA translation with sequencing

Sequence ribosome-protected mRNA

Predict protein abundance

Investigate translational control

Measure gene expression

Rapid, scalable spin-column method

No ultracentrifuge required

Compat
IBL
e with yeast and mammalian samples


Sequence actively translated transcripts

Ribosome profiling is a technique for investigating translational control that provides a snapshot of all the ribosomes active in a cell at a specific time. TruSeq Ribo Profile generates RNA-Seq libraries from ribosome-protected fragments of mRNAs that are actively being translated.


Use TruSeq Ribo Profile to sequence mRNA fragments undergoing translation by ribosomes. These mRNA fragments are called "footprinted" or ribosome-protected mRNA fragments.

You can also identify proteins being actively translated from samples prepared with TruSeq Ribo Profile. Samples collected at different times often show changes in translation. Samples treated with different drugs often show different translation patterns.
Specifications:
Input Quantity
0.1 – 1 ug high-quality purified total RNA from blood
Content Specifications
Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action
Bead-based rRNA depletion,
CDN
A synthesis, and PCR
Multiplexing
Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples
System Compatibility
NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500
Automation Cap
ABI
lity
Liquid Handling Robots
Variant Class
Transcript Variants
Species Category
Rat