商品编号


20005615



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
TruSeq Small RNA Library Preparation Kits provide reagents to generate small RNA libraries directly from total RNA. MicroRNAs (miRNAs) generated by Dicer processing are efficiently targeted by the included modified adapters.

These kits enable multiplexed sequencing with the introduction of 48 unique indexes, allowing miRNA and small RNA discovery and profiling throughput to match the unparalleled output of
Illumina
sequencing. Indexes are added in a universal amplification reaction, greatly reducing ligation bias and ensuring accurate measurement of miRNA expression.

Workflow improvements enable streamlined sample preparation, allowing economical studies covering all small RNA transcripts in any species. Compat
IBL
e applications include finding novel miRNAs, characterizing variation such as isomers with single-base resolution, and analyzing differential expression without prior assumptions.


TruSeq Small RNA Sample Datasets

The Human Brain Reference RNA (HBRR) and Universal Human Reference RNA (UHRR) samples were prepared using the TruSeq Small RNA Library Prep Kit. These libraries were sequenced on the MiniSeq System using the MiniSeq High Output Reagent Kit at a 1 x 36 bp read length configuration. The total yield was 0.91 Gb with 97% of bases at or above Q30.

Browse the Data In BaseSpace Sequence Hub:


View Run (primary analysis and metrics)

View Project (secondary analysis with BaseSpace Apps)

Specifications:
Assay Time
~10.5 hours
Hands-On Time
~30 minutes
Input Quantity
25 to 100 ng total RNA from species with polyA tails
Content Specifications
Captures the coding transcriptome with strand information
Mechanism of Action
Oligo-dT beads capture polyA tails
Multiplexing
Up to 24-plex pooling with additional NeoPrep runs
Sample Throughput
Up to 16 samples per NeoPrep library card
Species Category
Drosophila,Any Species,Mouse,Zebrafish,Human,Rat,Plant,Nematode
System Compatibility
MiSeq,NextSeq 550,MiniSeq,NextSeq 500
Specialized Sample Types
FFPE,Low Input
Technology
Sequencing
Automation Cap
ABI
lity
Liquid Handling Robots
Variant Class
Novel Transcripts,Transcript Variants
Method
miRNA and Small RNA Sequencing