商品编号


RS-122-2301



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:


The TruSeq Stranded Total Library Preparation kit provides a clear view of the transcriptome with a streamlined, cost-efficient, and scalable solution for total RNA analysis with sequencing.

Whole-Transcriptome Analysis with Precise Measurement of Strand Orientation

Compat
IBL
e with a wide range of samples, including low-quality and FFPE, TruSeq Stranded Total RNA kits couple all of the benefits of TruSeq RNA preparation kits with Ribo-Zero ribosomal RNA reduction chemistry, providing analysis of coding and multiple forms of noncoding RNA with precise measurement of strand orientation, uniform coverage, and high-confidence discovery of features such as alternative transcripts, gene fusions, and allele-specific expression.

Find an up-to-date list of high-throughput automation vendors with robotic systems compat
IBL
e with this library preparation kit



Flex
IBL
e Solutions for a Range of Study Designs

The TruSeq Stranded Total RNA Library Prep kit is available in multiple versions to fit specific study design needs:


TruSeq Stranded Total RNA with Ribo-Zero Human/Mouse/Rat
removes cytoplasmic rRNA.

TruSeq Stranded Total RNA with Ribo-Zero Gold
removes both cytoplasmic and mitochondrial rRNA.

The total RNA kits are also compat
IBL
e with the new unique dual index kits,
TruSeq RNA Unique Dual Indexes
, which allows multiplexing of up to 96 samples.


Species Compatibility

The TruSeq Stranded Total Library Preparation kit is designed for human, mouse and rat sequences, but may be applicable to a variety of eukaryotic species.*

*For information on a particular species of interest, contact
Illumina
Technical Support.
Specifications:
Input Quantity
0.1 – 1 ug high-quality total RNA. Lower-quality samples may require further optimization.
Content Specifications
Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action
Bead-based rRNA depletion,
CDN
A synthesis, and PCR
Multiplexing
Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kits: Prepare 96 uniquely indexed samples.
System Compatibility
NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 550,HiSeq 3000,HiSeq 1000,HiSeq 1500,NextSeq 500,HiSeq 2500,NovaSeq 6000,HiSeq 4000
Variant Class
Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Novel Transcripts,Transcript Variants
Specialized Sample Types
FFPE
Method
Whole-Transcriptome Sequencing
Species Category
Mouse,Human,Rat
Technology
Sequencing
Automation Cap
ABI
lity
Liquid Handling Robots