商品编号


RS-122-2501



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
TruSeq Stranded Total RNA with Ribo-Zero Globin kits deliver a clear and comprehensive view of the transcriptome from blood-derived RNA, with a fast, efficient sequencing library preparation workflow.

High-quality whole-transcriptome analysis with precise strand information

These kits couple the benefits of Ribo-Zero ribosomal RNA reduction chemistry with RNA sequencing (RNA-Seq) technology for whole-transcriptome analysis of human, mouse, or rat samples. This technology provides:


Uniform coverage and precise measurement of strand orientation

High-confidence discovery of alternative transcripts, antisense expression, and allele-specific expression

Discovery across both coding and multiple forms of non-coding RNA



Efficient ribosomal RNA and globin mRNA removal in a single step

Whole-transcriptome analysis of blood-derived RNA requires the removal of two forms of abundant RNA—ribosomal RNA (both cytoplasmic and mitochondrial) as well as globin mRNA, which is present in high levels in whole blood.

Tr
ADI
tional removal methods require two independent steps. This means the need for additional reagents, a longer workflow, and more input RNA lost. TruSeq Stranded Total RNA with Ribo-Zero Globin leverages Ribo-Zero chemistry to efficiently remove both forms of abundant RNA in a single, rapid step.

Find an up-to-date list of high-throughput automation vendors with robotic systems compat
IBL
e with this library preparation kit
Specifications:
Input Quantity
0.1 – 1 ug high-quality purified total RNA from blood
Content Specifications
Captures coding RNA plus multiple forms of non-coding RNA
Mechanism of Action
Bead-based rRNA depletion,
CDN
A synthesis, and PCR
Multiplexing
Low-throughput kits: Pool up to 12 samples. Or pool up to 24 samples with sets A and B together,High-throughput kit version: Prepare 96 uniquely indexed samples
System Compatibility
NovaSeq 5000,Genome Analyzer IIx,HiSeq 2000,NextSeq 500,HiSeq 2500,NextSeq 550,HiSeq 3000,HiSeq 1000
Automation Cap
ABI
lity
Liquid Handling Robots
Variant Class
Transcript Variants,Single Nucleotide Polymorphisms (SNPs),Gene Fusions
Species Category
Rat,Mouse
Method
Whole-Transcriptome Sequencing
Technology
Sequencing