商品编号


RS-122-2401



品牌


宜曼达(Illumina)



公司


Illumina, Inc.



公司分类


Library Preparation Kits


商品信息

Product Highlights:
High-Quality Whole-Transcriptome Analysis with Precise Strand Information

RNA-Seq technology provides uniform coverage, precise measurement of strand orientation and high-confidence discovery of features such as alternative transcripts, antisense expression and allele-specific expression across both coding and multiple forms of noncoding RNA. The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit couples the benefits of TruSeq RNA library preparation with Ribo-Zero ribosomal RNA reduction chemistry, providing a robust and scalable solution for whole-transcriptome analysis.

Find an up-to-date list of high-throughput automation vendors with robotic systems compat
IBL
e with this library preparation kit


Efficient Ribosomal RNA Reduction Across a Range of Plant Species, Study Designs

The TruSeq Stranded Total RNA with Ribo-Zero Plant Kit enables rapid and specific removal of cytoplasmic, mitochondrial and chloroplast ribosomal RNA from leaf, seed, and root tissue. The kit has been validated for use in multiple plant species, including Ar
ABI
dopsis thaliana, rice, and maize, and will provide efficient rRNA removal in a broad range of additional species.*

*For information on a particular species of interest, contact
Illumina
Technical Support.
Specifications:
Assay Time
~6.5 hours
Hands-On Time
~3.5 hours
Input Quantity
10 ng high-quality genomic DNA; 10 – 100 ng FFPE DNA (depending on QC results)
Mechanism of Action
Probe hybridization, extension-ligation, and PCR
Multiplexing
1–96
Content Specifications
Design custom probes to sequence genomic regions of interest. Content range: 4 – 650 kb
Variant Class
Short Tandem Repeats (STRs),Single Nucleotide Polymorphisms (SNPs),Gene Fusions,Loss of Heterozygosity (LOH),Somatic Variants,Chromosomal Abnormalities,Germline Variants,Structural Variants,Insertions-Deletions (indels)
System Compatibility
HiSeq 2000,MiSeq,NextSeq 550,HiSeq 3000,HiSeq 1000,MiSeqDx in Research Mode,MiniSeq,HiSeq 1500,NextSeq 500,MiSeq FGx in Research Mode,HiSeq 2500,HiSeq 4000
Species Category
Other,Bovine,Mouse,Human,Rat
Specialized Sample Types
FFPE,Low Input
Technology
Sequencing
Automation Cap
ABI
lity
Liquid Handling Robots
Method
Amplicon Sequencing,Custom Sequencing