商品编号


KK4964



品牌


Kapa biosystems



公司


Kapa Biosystems.



公司分类


Sample QC



Size

5 x 300 ?L





商品信息

Description:
DNA Standards (1 - 5) only
KAPA hgDNA Quantification and QC Kit

Quality matters.

KAPA hgDNA Quantification and QC Kits contain all the reagents needed for the qPCR-based quantification and quality assessment of human genomic DNA samples prior to NGS library construction.

Kits contain KAPA SYBR
?
FAST qPCR Master Mix, optimized for high-performance SYBR Green I-based qPCR, as well as a pre-diluted set of DNA standards and primer premixes targeting different portions of a highly conserved single-copy human locus. Absolute quantification is achieved with the primer pair defining the shortest fragment, whereas the additional primers are used to derive information about the amount of amplifiable template in the DNA sample. Quality scores (or Q-ratios) generated with the kit may be used to predict the outcome of library construction, or tailor workflows for samples of variable quality, particularly FFPE DNA.

The method is easy to automate and can be applied to any process or workflow that requires accurate quantification of dilute DNA samples, or samples that may contain a high proportion of DNA that is recalcitrant to PCR amplification.

Download our?KAPA hgDNA Quantification and QC Data Analysis Template.

For Research Use Only. Not for use in diagnostic procedures.

Product Highlights





Obtain concentration and quality information with a single assay


Allows for accurate quantification of dilute DNA samples

Quantification with an additional primer pair provides a Q-ratio that is indicative of sample quality







Employ quality scores?in the analysis of NGS library construction workflows


Q-ratios can provide valuable insights into the bottlenecks in NGS library construction workflows

For FFPE samples, library and sequence quality is primarily limited by inefficient conversion of input DNA to adapter-ligated library







0.4 yielded libraries that met minimum sequence quality requirements. Data on file.">


0.4 yielded libraries that met minimum sequence quality requirements. Data on file.">


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Obtain actionable data for sample preparation


Q-ratios correlate with key sequencing metrics such as duplication rates and mean target coverage

FFPE samples with a Q score >0.4 yield libraries of acceptable quality when processed in standard sample preparations for target capture





Applications:
Applications


FFPE samples

Free-circulating DNA from plasma or serum

Samples obtained by laser-capture microdissection of fresh, frozen or FFPE tissue

Forensic samples

Cells collected by flow cytometry

Any other limiting or precious clinical sample



Kit Specifications and Contents / Storage:
Kit Specifications and Contents / Storage

Kits can be stored for up to 12 months?at -20?C.

Complete (Master Mix) kits include KAPA SYBR FAST qPCR Master Mix (2X), Primer Premix (41 bp, 129 bp and 305 bp, 10X) and a set of 5 DNA Standards. Primer Premixes and DNA Standards are also sold separately.

Components




Specifications



Spec

Description



Compat
IBL
e Platform

All NGS platforms



Compat
IBL
e Samples

Human genomic DNA



Sample sources

FFPE tissue Cells collected by laser-capture microdissection Flow-sorted cells Free-circulating DNA from plasma or serum Forensic samples Clinical samples



Standard curve concentration range

2.5 ng/?L–10 pg/?L or 3,080–12 copies



Sequencing Applications

Whole Genome Sequencing Whole Exome Sequencing Targeted Sequencing (custom panels) Amplicon Sequencing