商品编号


KK8513



品牌


Kapa biosystems



公司


Kapa Biosystems.



公司分类


DNA Library Preparation



Size

24 reactions















商品信息

Description:
PCR-Free

KAPA HyperPlus Kits


NGS library preparation.? Evolved.

The KAPA HyperPlus kit provides a streamlined workflow that includes fragmentation and library preparation in a single tube. Building on industry-le
ADI
ng library construction efficiencies, this integrated solution combines enzymatic fragmentation, similar in quality to mechanical shearing, with the speed and convenience of tagmentation-based workflows. Benefits include:


DNA fragmentation and library prep in 2.5 hours*

Flex
IBL
e DNA sample input from 1 ng – 1 ?g*

Reduced bias and maximize sequence coverage*

PCR-free workflows


NEW!
KAPA Dual-Indexed Adapter Kits are now available. For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter and Bead Calculator.

Download our?Adapter and Bead Calculator

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*Data on file.
For Research Use Only. Not for use in diagnostic procedures.

KAPA HyperPlus Workflow

Product Highlights











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Tunable and reproduc
IBL
e fragmentation


Adjust library insert sizes from 150 – 800 bp by varying fragmentation time

Reproduc
IBL
e insert sizes across a range of GC content and DNA input amounts







Industry le
ADI
ng library yields…


Routinely achieve conversion rates >80% from ≥ 100 ng input*

Superior performance across a range of DNA input amounts*

High library yields enable PCR-free workflows from as little as 50 ng starting material*
















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…Enable s
uperior sequencing r
esults


Higher conversion rates results in fewer amplification cycles and lower duplication rates

Detect low-frequency mutations with high confidence due to greater library diversity and more uniform sequence coverage
















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Minimal sequence coverage bias


Lower sequence bias when compared to tagmentation and other enzymatic fragmentation methods*

Equivalent performance to mechanical shearing*

Less bias leads to more uniform sequencing coverage and reduced sequencing costs*


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*Data on file.





Related Products

Are you sequencing low-input, FFPE or high quality DNA? ?RNA? ?Check out these Kapa NGS products to improve your workflow and results:

KAPA hgDNA Quantification and QC Kit

KAPA Library Amplification Kits

KAPA Library Quantification Kits

KAPA Stranded mRNA-Seq Kits




Applications:
Applications


Whole Genome Sequencing

Whole Exome Sequencing

Targeted Sequencing (custom panels)

FFPE Sample Sequencing



Kit Specifications and Contents / Storage:
Kit Specifications and Contents / Storage

Kits can be stored for up to 6 months at -20?C.

Kits contain KAPA Frag Enzyme, KAPA Frag Buffer (10X), KAPA Frag Conditioning Solution, KAPA End Repair & A-Tailing Buffer,?KAPA End Repair & A-Tailing Enzyme,?KAPA Ligation Buffer,?KAPA DNA Ligase,?KAPA HiFi HotStartReadyMix and?KAPA Library Amplification Primer Mix.

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Specifications



Spec

Description



Compat
IBL
e Platform

llumina HiSeq?, MiSeq?, NextSeq?



Library Type

DNA



Starting Material

genomic DNA, FFPE DNA, PCR amplicons



Input Amount

1 ng–1 ?g