商品编号


KK8401



品牌


Kapa biosystems



公司


Kapa Biosystems.



公司分类


RNA Library Preparation



Size

96 libraries









商品信息

Description:
Stranded RNA-Seq Kit

KAPA Stranded mRNA-Seq Kits


Even difficult messages should be understood.

KAPA Stranded mRNA-Seq Kits include all the enzymes and buffers required for
CDN
A library preparation for
Illumina
Next-Generation Sequencing, utilizing 100 ng – 4 ?g of total RNA. KAPA mRNA Capture Beads are included for isolation of poly(A)-tailed RNA. Kits provide precise measurement of strand orientation (>99%), uniform coverage, and high-confidence mapping of alternate transcripts, and are optimized for the improved coverage of GC-rich and low-abundance transcripts.* Kits contain KAPA HiFi for high-efficiency and low-bias library amplification, as well as KAPA mRNA Capture Beads and a streamlined, “with-bead” protocol.

KAPA Stranded RNA-Seq Kits include all the enzymes and buffers required for
CDN
A library preparation for
Illumina
Next-Generation Sequencing, but do not contain the KAPA mRNA Capture Beads. Kits can be used to prepare libraries from 10 – 400 ng of either poly(A)-selected, ribosomally depleted, or total RNA.

NEW! KAPA Dual-Indexed Adapter Kits are now available.
For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter and Bead Calculator.

Download?Adapter and Bead Calculator

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*Data on file.

For Research Use Only. Not for use in diagnostic procedures.

Product Highlights





Uncover challenging transcripts?


Improved coverage of GC-rich transcripts

Enhanced identification of exonic regions







Detect low-abundance transcripts


Enables identification of transcripts missed by competitor kits, even with high input*

High uniformity across varying amounts of sample input*




30M), KAPA Stranded mRNA-Seq libraries generate a greater percentage of mapped reads and lower duplication rates than analogous libraries prepared with the Illumina Stranded mRNA Sample Prep Kit while maintaining 99% strand specificity. Data on file.">


Identify more genes


Higher percentage of uniquely mapped reads compared to
Illumina
?TruSeq? Stranded mRNA Sample Prep Kits*

Lower duplication rates yield better coverage







Maintain high coverage uniformity


Minimal 5’–3′ bias across transcripts

More uniform distribution of reads over each transcript


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*Data on file.





Related Products

Sequencing challenging RNA samples? How about DNA? Check out these Kapa NGS products to improve your workflow and results:

KAPA hgDNA Quantification and QC Kit

KAPA Library Amplification Kits

KAPA Hyper Prep Kits

KAPA Library Quantification Kits




Applications:
Applications


Gene expression

Single nucleotide variation (SNV) discovery

Post-transcriptional SNVs

Fusion gene identification

Targeted transcriptome

Whole transcriptome



Kit Specifications and Contents / Storage:
Kit Specifications and Contents / Storage

Kits can be stored for up to 8?months?at -20?C.?KAPA mRNA Capture Beads can be stored for up to 8 months?at -4?C.

Components




Specifications



Spec

Description



Compat
IBL
e Platform


Illumina
HiSeq, NextSeq, MiSeq and GAIIx



Starting Material

Total RNA, mRNA, or ribosomal depleted RNA



Input Amount

Stranded mRNA-Seq Kits: 100 ng–4 ?g Stranded RNA-Seq Kits: 10 ng–400 ng