商品编号


KK8000



品牌


Kapa biosystems



公司


Kapa Biosystems.



公司分类


RNA Library Preparation



Size

5 mL


















商品信息

Description:
KAPA Pure Beads (5 mL)
KAPA Stranded RNA-Seq with RiboErase

Evolved to focus.

The KAPA Stranded RNA-Seq Kit with RiboErase offers a high-quality, comprehensive solution for transcriptome sequencing. By utilizing a targeted enzymatic method for depletion, our workflow enables superior reduction of ribosomal RNA (rRNA) and a more complete representation of the transcriptome, including precursor mRNAs and non-coding RNA (ncRNA). Kits also contain KAPA HiFi for high-efficiency and low-bias library amplification, and include a streamlined, “with-bead” protocol.


Up to 99.98% rRNA depletion*

Flex
IBL
e input of 100 ng – 1 μg total RNA from human, mouse, or rat species*

Robust and reproduc
IBL
e across various input amounts

Automation-friendly workflow


NEW! KAPA Dual-Indexed Adapter Kits are now available.
For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter and Bead Calculator.

Download our Adapter and Bead Calculator

?

*Data on file.

For Research Use Only. Not for use in diagnostic procedures.

Product Highlights











Prev Next


Industry le
ADI
ng rRNA depletion


Superior rRNA depletion from high-quality and FFPE samples*

More economical NGS sequencing due to decreased rRNA reads, providing deeper sequencing of transcripts of interest
















Prev Next


Unsurpassed sequencing data quality


Detection of more genes and unique transcripts

Accurate and clear identification of splice sites and alternative gene splicing

Improved coverage enabling better detection of difficult and GC-rich transcripts










0.973) which are better than libraries prepared from the equivalent kits from Illumina or NEB. Data on file.">


0.973) which are better than libraries prepared from the equivalent kits from Illumina or NEB. Data on file.">


0.973) which are better than libraries prepared from the equivalent kits from Illumina or NEB. Data on file.">


Prev Next


Highly reproduc
IBL
e sequencing results


High correlation even between different testing conditions

Low sample-to-sample variation for more reliable results













Prev Next


Improved coverage uniformity


Uniform distribution of reads over each transcript

Minimal 5’ – 3’ bias across transcripts


*Data on file.





Related Products

Are you sequencing low-input, FFPE or high quality DNA? ?RNA? ?Check out these Kapa NGS products to improve your workflow and results:

KAPA Stranded mRNA-Seq Kits

KAPA RNA HyperPrep Kits with RiboErase

KAPA mRNA HyperPrep Kits

KAPA Library Amplification Kits

KAPA Hyper Prep Kits

KAPA Library Quantification Kits




Applications:
Applications


Whole Transcriptome

Gene Expression

Transcript Discovery and Annotation

Total RNA-Seq



Kit Specifications and Contents / Storage:
Kit Specifications and Contents / Storage

Kits can be stored for up to 10 months at -20?C.

Kits contain KAPA Hybridization Buffer, KAPA Hybridization Oligos (Human/Mouse/Rat), KAPA Depletion Buffer, KAPA RNase H, KAPA DNase Buffer, KAPA Dnase, KAPA Fragment, Prime and Elute Buffer (2X), KAPA 1st Strand Synthesis Buffer, KAPA Script, KAPA 2nd Strand Marking Buffer, KAPA 2nd Strand Synthesis Enzyme Mix, KAPA A-Tailing Buffer (10X), KAPA A-Tailing Enzyme, KAPA Ligation Buffer (5X), KAPA DNA Ligase, KAPA PEG/NaCl SPRI Solution, KAPA Library Amplification Primer Mix (10X) and KAPA HiFiHotStartReadyMix (2X).

Components




Specifications



Spec

Description



Compat
IBL
e Platform


Illumina
HiSeq?, NextSeq? and MiSeq?



Library Type

RNA



Starting Material

High-quality Total RNA



Input Amount

100 ng - 1 ?g