商品编号


KK8721



品牌


Kapa biosystems



公司


Kapa Biosystems.



公司分类


RNA Library Preparation



Size

25 mL











商品信息

Description:
KAPA Adapter Dilution Buffer (25 mL)
KAPA mRNA HyperPrep Kits

Single-Day RNA

The KAPA mRNA HyperPrep Kits utilize novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality mRNA-seq libraries. mRNA capture beads are used prior to library preparation, which enriches for mature mRNA over non-polyadenylated species, such as ribosomal, precursor, and noncoding RNAs.

The strand-specific workflow is flex
IBL
e – supporting library construction from lower-input amounts and degraded samples. Kits contain all reagents required for mRNA capture and library preparation, with the exception of KAPA Adapters (available separately). Benefits include:


single-day library construction, inclusive of mRNA capture

reduced hands-on and overall time through fewer enzymatic and reaction cleanups

flex
IBL
e input of 50 ng – 1 ?g total RNA*

maintain over 99% strand specificity*

KAPA Pure Beads included for reaction purifications



KAPA RNA HyperPrep Kits

KAPA RNA HyperPrep Kits leverage the same
CDN
A synthesis and library preparation workflow improvements as outlined for the KAPA mRNA HyperPrep Kits. KAPA RNA Hyper Prep Kits include all enzymes and buffers required for
CDN
A library preparation, but do not contain mRNA capture reagents or KAPA Adapters. Kits can be used to prepare libraries from 1 ng – 100 ng of total, poly(A)-selected, or rRNA-depleted RNA.


NEW! KAPA Dual-Indexed Adapter Kits are now available.
?For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter?and Bead Calculator.

Download our Adapter and Bead Calculator

?

*Data on file.
For Research Use Only. Not for use in diagnostic procedures.

Product Highlights











Prev Next


Sequence what matters


Waste fewer reads due to the combination of rRNA carryover and PCR duplicates

Identify more unique transcripts and genes with equivalent sequencing













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Achieve superior coverage uniformity


Obtain more uniform distribution of reads across transcripts

Improve coverage of difficult GC-rich transcripts






Related Products

Are you sequencing low-input, FFPE or high quality DNA? ?RNA? ?Check out these Kapa NGS products to improve your workflow and results:

KAPA RNA HyperPrep Kits with RiboErase

KAPA Stranded mRNA-Seq Kits

KAPA Library Amplification Kits

KAPA Hyper Prep Kits

KAPA Library Quantification Kits




Applications:
Applications


Gene expression analysis

Detection of gene fusions, isoforms, and other structural variants

Novel transcript identification, including noncoding transcripts

SNV discovery

Whole transcriptome (KAPA RNA Hyper Prep Kits only)

Targeted transcriptome (KAPA RNA Hyper Prep Kits only)



Kit Specifications and Contents / Storage:
Kit Specifications and Contents / Storage

Enzymes and buffers for rRNA depletion,
CDN
A synthesis, and library preparation can be stored for up to 10 months at -20°C. mRNA capture reagents and KAPA Pure Beads can be stored for up to 10 months at 4°C.
(US only)

Kits include reagents for RNA fragmentation,
CDN
A synthesis, and library preparation. Kits with reagents for mRNA capture are also available.




Specifications



Spec

Description



Compatibility Platform


Illumina
HiSeq, NextSeq, and MiSeq



Library Type

RNA



Starting Material

RNA HyperPrep Kit: High-quality total RNA, mRNA, or rRNA-depleted RNA; mRNA HyperPrep Kit: High-quality total RNA



Starting Material

RNA HyperPrep Kit: 1ng – 100 ng; mRNA HyperPrep Kit: 25 ng – 1 ?g