商品编号


KK8712



品牌


Kapa biosystems



公司


Kapa Biosystems.



公司分类


RNA Library Preparation



Size

12 adapters x 40 ?l each
















商品信息

Description:
KAPA Single-Indexed Adapter Kit, Set B (1.5 ?M)
KAPA RNA HyperPrep Kits with RiboErase (HMR)

Single-Day RNA

The KAPA RNA HyperPrep Kits with RiboErase (HMR) utilize novel chemistry that enables the combination of enzymatic steps and fewer reaction purifications, resulting in a truly streamlined solution for the preparation of high-quality rRNA-depleted RNA-seq libraries. By utilizing a targeted enzymatic method for depletion, our workflow enables efficient reduction of ribosomal RNA (rRNA) and a more complete representation of the transcriptome, including precursor mRNAs and non-coding RNA (ncRNA). The strand-specific workflow is flex
IBL
e – supporting library construction from lower-input amounts and degraded samples. Kits contain all reagents required for rRNA depletion and library preparation, with the exception of KAPA Adapters (available separately). Benefits include:

single-day library construction, inclusive of rRNA depletion

reduced hands-on and overall time through fewer enzymatic and reaction cleanups

flex
IBL
e input of 25 ng – 1 ?g total RNA from human, mouse, or rat species*

higher success rates with lower input and degraded samples

maintain over 99% strand specificity*

KAPA Pure Beads included for reaction purifications


NEW! KAPA Dual-Indexed Adapter Kits are now available.
?For more information on KAPA Adapter Kits, scroll down to the Ordering section, or download the KAPA Adapter and Bead Calculator. Download our Adapter and Bead Calculator

?

*Data on file.

For Research Use Only. Not for use in diagnostic procedures.


Product Highlights











Prev Next


Sequence what matters


Waste fewer reads due to the combination of rRNA carryover and PCR duplicates

Identify more unique transcripts and genes with equivalent sequencing













Prev Next


Achieve superior coverage uniformity


Obtain more uniform distribution of reads across transcripts

Improve coverage of difficult GC-rich transcripts













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Generate high-quality libraries from degraded samples


Input as little as 25 ng with FFPE samples, depending on total RNA quality

Achieve low duplication rates and highly efficient, reproduc
IBL
e rRNA removal with degraded samples

Identify more unique transcripts and genes with equivalent sequencing
















Prev Next


Achieve reliable results with degraded inputs


Attain a high-degree of expression correlation between paired FFPE and fresh frozen samples which provides increased confidence in sequence data accuracy






Related Products

Are you sequencing low-input, FFPE or high quality DNA? ?RNA? ?Check out these Kapa NGS products to improve your workflow and results:

KAPA mRNA HyperPrep Kits

KAPA Stranded mRNA-Seq Kits

KAPA Library Amplification Kits

KAPA HyperPrep Kits

KAPA Library Quantification Kits




Applications:
Applications


Whole transcriptome

Gene expression analysis

Detection of gene fusions, isoforms, and other structural variants

Novel transcript identification, including noncoding transcripts

SNV discovery



Kit Specifications and Contents / Storage:
Kit Specifications and Contents / Storage

Enzymes and buffers for rRNA depletion,
CDN
A synthesis, and library preparation can be stored for up to 10 months at -20°C. KAPA Pure Beads can be stored for up to 10 months at 4°C.
(US only)

Kits contain Hybridization Buffer, Hybridization Oligos (HMR), Depletion Buffer, RNase H, DNase Buffer, DNase, Fragment, Prime and Elute Buffer (2X), 1stStrand Synthesis Buffer, KAPA Script, 2nd Marking Buffer, 2nd Strand Synthesis & A-Tailing Enzyme Mix, Ligation Buffer, DNA Ligase, PEG/NaCl Solution, KAPA Pure Beads, Library Amplification Primer Mix (10X), and KAPA HiFi HotStart ReadyMix (2X).




Specifications



Spec

Description



Compat
IBL
e Platform

0616



Library Type

RNA



Starting Material

High-quality and degraded total RNA



Input Amount

25 ng – 1 ?g