商品编号


TOPOA -50A



品牌


MCLab



公司


MCLAB.Inc



公司分类


NGS Library Preparation Kit



Description

24 samples, 24 Indexes


Size

1 Ea

商品信息


MCLAB
’s Topomize Amplicon Library Prep Kit is an innovative, fast and high quality Amplicon library construction kit for next generation sequencing.




Description:


MCLAB
’s Topomize Amplicon Library Prep Kit is an innovative, fast and high quality Amplicon library construction kit for next generation sequencing. Targeted sequencing of large cohorts of samples is a powerful tool for the discovery and detection of disease-causing variants associated with many inherited diseases and cancers. After PCR amplification of the targeted DNA regions to generate amplicon pools,
MCLAB
’s Topomize Amplicon Library Prep Kit can be used to quickly add adapters with barcodes for deep sequencing. ?Instead of using tr
ADI
tional ligase-based methods, our kit uses topoisomerase-based technology to attach adapters with barcodes onto the amplicons. The Topomize Amplicon Library Kit has unparalleled efficiency and yield with no adapter dimers or chimeras. The kit is designed for 10ng to 1?g of each pooled amplicon DNA input, and is compat
IBL
e with the
Illumina
platforms. The fast workflow allows the amplicon library to be ready in less than 40 minutes.
?


Highlights:



Easy and cost saving primer design
: no linkers or extra nucleotides are needed except the target region primers


More specific amplifications
: primers are 100% matched to the target regions


Versatility
: adapters with barcode can be attached to any blunt end PCR products


Unbeatable efficiency
: Topoisomerase based enzymology generates higher adapter attachment rate with no bias


High quality
: no adapter dimer or other chimeras


Fast workflow
: get your amplicon library ready in less than 40 min


All-in-One kit
: includes all the reagents for sample prep and compat
IBL
e with
Illumina
platforms


Figure:


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Topomize Amplicon Library Prep Workflow