商品编号


MNEXT-4



品牌


MCLab



公司


MCLAB.Inc



公司分类


NGS Library Preparation Kit



Description

4 Samples, Includes Index Kit (4 Indices)


Size

1 Ea


商品信息

The MCNext DNA Sample Prep Kit is a high performance, cost-effective sample prep solution for NGS using the
Illumina
platforms.




Description


Kit Features

The MCNext DNA Sample Prep Kit is a high performance, cost-effective sample prep solution for NGS using the
Illumina
platforms. A bead-based, gel-free size selection workflow in only 90 min eliminates the need for mechanical fragmentation and agarose gels in size selection, the avail
ABI
lity of pair-ends 96 indices facilitates multiplex high-throughput applications. The MCNext DNA Sample Prep Kit incorporates different flanking sequence additions by Dialatum
TM
transposome (US patent pending), resulting in fewer primer-dimers and a better signal/noise ratio in sequencing outcomes.



Fast Prep Workflow

Complete the protocol in 90 minutes

Enzymatic fragmentation without sonication

One-step fragmentation/tagging to save time??

?


Low Input and High Throughput

Maximum of 50 ng input DNA needed

Pre-mixed enzymes and nucleotides for multiplexed processing

Plate-based processing compat
IBL
e for up to 96 samples per kit

?


Higher Coverage Rate and Full Compatibility

Directional transposome doubles the sequencing coverage

Works on all
Illumina
NGS platforms


?


Best Value on Market

All 96
Illumina
indices included



Figure 1. MCNext
TM
Sample Preparation Kit workflow takes 90 min to finish the sample prep.



Figure 2. MCNext
TM
Sample Preparation Kit fragments and directionally tags DNA in a single step to boost sequencing coverage and signal strength. Subsequent limited-cycle PCR amplification adds dual indices to enable up to 96 libraries to be pooled and sequenced.


Kit Compatibility


Illumina
Genome Analyzer IIx

Illumina
?HiScanSQ

Illumina
?HiSeq 1000

Illumina
?HiSeq 2000

Illumina
?HiSeq 2500

Illumina
?MiSeq Desktop Sequencer v1, v2 and v3

Illumina
?NextSeq 500 Sequencer

Questions & Answers


Q: What are the main protocol differences between the MCNext DNA Sample Prep Kit and the conventional NGS DNA Sample Prep kit?

A: The MCNext DNA Sample Prep kit utilizes a single-well enzymatic reaction for both fragmentation and adapters addition for library construction, so there is no mechanical fragmentation/shearing required for DNA sample prep. The whole streamlined sample prep process only takes 90 min to complete comparing to a whole day workflow of ?the conventional NGS preps.



Q: What are the similarities between the MCNext DNA Sample Prep Kit and the
Illumina
Nextera DNA Sample Prep Kit?

A: Both kits utilize enzymatic fragmentation without sonication and speed up library construction by one step fragmentation/tagging; both kits require low DNA input with 50ng range; both kits are optimized for multiplexing up to 96 samples and both kits take as short as 90 min to complete the sample prep.



Q: What are the advantages of the MCNext DNA Sample Prep Kit comparing with
Illumina
Nextera DNA Sample Prep Kit?

A: MCNext DNA Sample Prep Kit provides the Dialatum
TM
transposome (US patent pending) in the tagmentation step with different flanking sequences added prior to the P5-Read1/Read2-P7 addition. This flanking sequence differentiation significantly enhances the library construction efficiency, meanwhile, minimizes primer-dimer formation comparing with the unilateral adapter addition by
Illumina
Nextera DNA Sample Prep Kit, which allows more PCR cycles in the subsequent amplification step for a better signal/noise ratio. ?The other advantage is that the MCNext DNA Sample Prep Kit contains all sequencing indices for
Illumina
platforms, an extra saving for customers.?



Q: What are the additional components I need to order to complete the prep?

A: ?Since the
Illumina
sequencing indices are all included in this kit, customers only need to order the Zymo? Purification Kit, the
Beckman
Coulter Agencourt
AMPure XP
60 ml Kit, ?and some general lab consumables described in the user manual.



Q: Are kit components such as primer mix and buffers interchangeable between MCNext DNA Sample Prep Kit and
Illumina
Nextera DNA Sample Prep Kit?

A:? No. The reagents are not interchangeable because in the tagmentation step the Dialatum
TM
transposome has a different oligo insertion and the
Illumina
transposome produces a unilateral adapter addition, the library products differ from the very beginning of the sample prep, even the workflows are similar, but the chemistry is different downstream.



Illumina
? is a registered trademark of
Illumina
, Inc.