商品编号


NGRR-300?



品牌


MCLab



公司


MCLAB.Inc



公司分类


NGS Library Preparation Kit



Description

48 reactions


Size

1 Ea

商品信息


MCLAB
's RNA-Seq Library Construction Kit is a highly efficient library construction kit for preparing single, paired-end or multiplexed
CDN
A libraries for high-throughput sequencing.



Description:

MCLAB
's RNA-Seq Library Construction Kit is a highly efficient library construction kit for preparing single, paired-end or multiplexed
CDN
A libraries for high-throughput sequencing. This kit can be used to convert RNA transcripts into
CDN
A whole transcriptome libraries or small RNA libraries for next-generation sequencing analysis using
Illumina
?
GAIIx
TM
, HiSeq
TM
2000 and MiSeq
?
sequencing platforms. With preserving the complexity of sequencing libraries, the protocol is quicker than the standard method and could be used routinely for RNA sequencing through the
Illumina
?
platform.
Function:
The superior function of
MCLAB
's RNA-Seq Library Construction Kit depends on our proprietary enzyme systems. Unique improvement to each key enzyme at specific steps in the library construction workflow increases sensitivity, flexibility and speed for next-generation sequencing. The target RNA fragments can be tagged with linkers at both ends by RNA ligases (Cat# T4RL1-100 and T4RL2T-100). Corresponding
CDN
A can be synthesized by Universal Reverse Transcriptase (Cat# SSII-100). The
CDN
A library can be enriched by PCR with high fidelity Pfu DNA Polymerase (Cat# AD-200). After gel- or beads-based purification for size selection, final
CDN
A libraries can hybridize directly to the oligonucleotides on the flow cell surface for cluster generation and sequencing thereafter.
Features:
- Simplified workflow, reducing hands-on time
- Optimized reaction conditions, increasing sensitivity
- Straightforward protocol minimizing prior experience needed
- Strand-specific sequencing data
- Cost-effective with all key enzymes
- High flexibility: multiplex samples and automation friendly
- Functionally validated with
Illumina
?
GAIIx
TM
, HiSeq
TM
2000 and MiSeq
?
sequencing platforms
Recommended Storage Conditions:
-20°C.