商品编号


LVP412-PBS



品牌


Gentarget



公司


Gentarget



公司分类


CRE recombinase




1 Ea

商品信息


Pre-made lentiviral particles
bicistronically
express two
individual
proteins:
firefly luciferase
and
nuclear permeable
CRE?recombinase
,
under the same suCMV promoter. A

GFP
-Puromycin
fusion dual
Marker
was expressed under RSV promoter, which allow to select the positive transduced stable cells for long term culture via
GFP cell sorting
or via
antibiotic selection
.

Both
lucifease
and
CRE
are highly active, best for both enzymes' assays
In vivo
or
In vitro
.
CRE
protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences,?named
lox sites
. CRE lentiviral particles?can?catalyze?the jointing event
In Vivo
?in the presence of?lox sites. This?
nuclear permeant?CRE (NLS-CRE)
?increases the recombination events
in vivo.
?Purified CRE enzyme can joint individual plasmids containing lox sites.


Luciferae
and NLS-
CRE
?are constitutively expressed in high level, and optionally, also can be used as tetracycline induc
IBL
e expression?as desired.
Luciferase
and
GFP
provide?the convenient, realtime monitoring?signals.???

This particles is concentrated and buffer changed into
PBS solution
, best suitable for hard-to-transduced cell lines, or serum-sensitive cell lines and in vivo application.

See
product manual
for details (.pdf).

Amount:??
200ul/per vial, at?5 x 10
7
IFU/ml in PBS
.?

Cat#: LVP412-PBS