商品编号


LVP1066



品牌


Gentarget



公司


Gentarget



公司分类


Target Expression




1 Ea

商品信息

This Pre-made optional induc
IBL
e lentiviral particles?expresses
mouse
?target,
Sgpl1?
(mouse sphingosine phosphate lyase 1)
?
(alternative name: AI428538; D10Xrf456; S1PL; Spl ). The sub-cloned codon sequence is identical (100% match) to CDS region ?in NCBI ID:
?NM_001316673.1.?

The full?sequence-verified target is expressed with a C-terminal
V5 tag
?()AA seq:?
GKPIPNPLLGLDST?
) under
a
n optional induc
IBL
e?suCMV promoter
.?This lentivirus can?be
used for regular constitutive high expression (without any induction) or used for?
induc
IBL
e expression
?with tetracycline or Dox induction when the tetracycline repressor protein (TetR) is present.

A?
Blasticidin
-
RFP
?
fusion dual
Marker
under the RSV promoter allows fluorescence sorting or blasticidin resistance selection of ?transduced cells. ?The RFP signal additionally provides convenient, real-time monitoring of the particles’ performance.

Each Lentivirus is validated on lot-by-lot basis,?and target expression is guaranteed. ?See
Product Manual

for details (.pdf).

Amount
: 200ul, at 1 x 10
7
IFU/ml in?DMEM medium with 10% FBS and 10x of polybrene (60 ug/ml). (
Note
: When specifically requested, the concentrated particles can be provided?at 1 x 10
8
IFU/ml in 200ul
PBS
solution for $650.00 USD).

C
a
t#: LVP1066