商品编号


LTSH-U6-GP



品牌


Gentarget



公司


Gentarget



公司分类


shRNA Lentivectors



Size

1 Ea

商品信息

pEco-Lenti-U6-shRNA-(
GFP
-Puro
) Cloning Kit
is designed to cloning of double-strand DNA encoding a short hairpin RNA. Once transcribed, the shRNA was processed into short RNA
in vivo
for RNAi analysis. To make shRNA expressing vector, two synthetic oligonucleotides were first annealed to form the double stand DNA duplex which was then cloned into the Ready-to-use, linear pEco-Lenti-U6-(GFP-Puro) vector via T4 enzyme ligation.

The transcription of shRNA was driven by a constitutive human U6 promoter, a RNA polymerase III promoter. This Kit provides materials for generating of your own shRNA expression lentivectors?with the following advanced features.? Please see
product manual
for details.

shRNA vector cloning scheme
:
? ? ?


Kit contents:

pEco-Lenti-U6-(
GFP
-
Puro
) linear vector:?????????????????10 ul (10rxn, 1ul/rxn)
10x shRNA oligo annealing solution:?????????????????????????50 ul
5X ligation buffer:????????????????????????????????????????????????? 50 ul
T4 ligase enzyme:????????????????????????????????????????????????? 10 ul (10rxn, 1ul/rxn)
Cloning control insert: annealed Luc-shRNA duplex:???5 ul (5 rxn, 1ul/rxn)
Sh-Sequencing primer:?????????????????????????????????????????? 10ul (10rxn, 1ul/rxn)

Material required but not included:
DH5a chemical competent cells:???????????????????????10 vials (10xn, 1 vial/rxn)


Key Features:





Linearized vector
and ready for use, no need for the tedious bench works for preparation of vector backbone;