商品编号


P7012-250MG



品牌


西格玛奥德里奇(Sigma-Aldrich)



公司


Merck & Co., Inc.



公司分类


Chemistry



Size

250MG





商品信息

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Synonym:

Pepsin A, Pepsin from hog stomach

CAS Number
9001-75-6



?EC Number
232-629-3



?Enzyme Commission (EC) Number
3.4.23.1??(?BRENDA
?|?IUBMB
?)



?MDL number
MFCD00081840



?eCl@ss
42010127



Popular?Documents:??Product Information Sheet (PDF)??|??Specification Sheet (PDF)

?

Properties:

Related Categories

3.4.x.x Peptidases, 3.x.x.x Hydrolases, Antibodies, Antibody Fragmentation, Antibody Modification,

Application Index, BRDU Cell Proliferation Assay Reagents, Biochemicals and Reagents, Cell
BIOLOG
y, Clinical Chemistry, Core Bioreagents, Core Bioreagents Enzymes, Enzyme Class Index, Enzymes for Diagnostic Kit Manufacturing, Enzymes, Inhibitors, and Substrates, Pepsin, Proteases, Proteases & Protein Sequencing, Proteolytic Enzymes, Proteolytic Enzymes and Substrates, Research Essentials, Selective Proteolytic Enzymes, Supplementary Products
More...



Quality Level ?

PREMIUM


material ?

off-white to beige


?

off-white to yellow


form ?

lyophilized powder


mol wt ?

mol wt 35?kDa


solubility ?

deionized water: soluble 10?mg/mL


?

10 mM HCl: soluble 4?mg/mL (Cold)


Featured Industry ?

Diagnostic Assay Manufacturing


storage temp. ?

?20°C


Gene Information ?

pig ... LOC396892(396892)

Description:
Analysis Note

E
1%/280
=14.7

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60?°C. Pepsin is irrevers
IBL
y inactivated at pH 8.0 - 8.5.

Protein determined by E
1%/280


Application

Pepsin cleavage can be used to produce F(ab′)
2
fragments of antibodies. pepsin at www.
Sigma-Aldrich
.com/enz
ymeexplorer.

Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice
.

The enzyme from Sigma has been used in the digestion of crude wheat gli
ADI
n.
It has been used along with other enzymes to demonstrate the effects of fixation and enzymatic digestion in immunohistochemical assays, using paraffin embedded tissue.
It has been used for digestion (before using immunoperoxidase techniques) to reduce non-specific background staining in sections of bronchial tissues.
The enzyme has also been used in the preparation of F(ab)
2
fragment from IgG.


Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Pepsin hydrolyzes peptide bonds, not amide or ester linkages. Pepsin cleaves peptides with an aromatic acid on either side of the peptide bond. Sulfur-containing amino acids increase susceptibility to hydrolysis when they are close to the peptide bond. Pepsin preferentially cleaves at the carboxyl side of phenylalanine and leucine and at the carboxyl side of glutamic acid residues. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin
Pepsin is the major proteolytic enzyme produced in the stomach. It digests proteins through the cleavage of interior peptide linkages.


The enzyme does not cleave at valine, alanine, or glycine linkages.
Z-L-tyrosyl-L-phenylalani
ne, Z-L-glutamyl-L-tyrosine, or Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion.
Pepsin is inhibited by several phenylalanine-containing peptides.


Other Notes

View more information on pepsin at www.
Sigma-Aldrich
.com/enz
ymeexplorer.

Packaging

1, 5, 10, 25, 100 g in poly bottle

250 mg in poly bottle

Unit Definition

One unit will produce a ΔA
280
of 0.001 per min at pH 2.0 at 37°C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16mL. Light path = 1cm.)
Safety:

Symbol?


??GHS07, GHS08



Signal word?


Danger




Hazard statements?

H315-H319-H334-H335



Precautionary statements?

P261-P305 + P351 + P338-P342 + P311



Personal Protective Equipment?

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



RIDADR?

NONH for all modes of transport



WGK Germany?

1



RTECS?

SC6132000