商品编号


P6887-250MG



品牌


西格玛奥德里奇(Sigma-Aldrich)



公司


Merck & Co., Inc.



公司分类


Chemistry



Size

250MG





商品信息

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Synonym:

Pepsin A, Pepsin from hog stomach

CAS Number
9001-75-6



?EC Number
232-629-3



?Enzyme Commission (EC) Number
3.4.23.1??(?BRENDA
?|?IUBMB
?)



?MDL number
MFCD00081840



?eCl@ss
42010127



Popular?Documents:??Product Information Sheet (PDF)??|??Specification Sheet (PDF)

?

Properties:

Related Categories

3.4.x.x Peptidases, 3.x.x.x Hydrolases, Antibody Characterization and Analysis, Application Index, Biochemicals and Reagents,

Clinical Chemistry, Core Bioreagents, Core Bioreagents Enzymes, Enzymatic and Chemical Protein Cleavage, Enzyme Class Index, Enzymes for Diagnostic Kit Manufacturing, Enzymes, Inhibitors, and Substrates, Mass Spectrometry, Molecular
BIOLOG
y, Pepsin, Proteases, Proteases & Protein Sequencing, Proteases for Cleavage of Proteins, Protein Sequencing, Proteins and Derivatives, Proteolytic Antibody Fragmentation, Proteolytic Enzymes, Proteolytic Enzymes and Substrates, Proteomics, Research Essentials, Selective Proteolytic Enzymes
More...



Quality Level ?

PREMIUM


material ?

white to off-white


form ?

lyophilized powder


mol wt ?

mol wt 35?kDa


impurities ?

salt, essentially free


solubility ?

deionized water: soluble 10?mg/mL


?

10 mM HCl: soluble 4.0?mg/mL (Cold)


Featured Industry ?

Diagnostic Assay Manufacturing


shipped in ?

wet ice


storage temp. ?

?20°C


Gene Information ?

pig ... LOC396892(396892)

Description:
Analysis Note

Optimum pH is 2-4. Active in 4 M urea and 3 M guanidine HCl. Stable at 60?°C. Pepsin is irrevers
IBL
y inactivated at pH 8.0 - 8.5.

Protein determined by E
1%/280


Application

Pepsin cleavage can be used to produce F(ab′)
2
fragments of antibodies. pepsin at www.
Sigma-Aldrich
.com/enz
ymeexplorer.

Pepsin from Sigma has been used along with other enzymes for the determination of enzyme-resistant starch (RS) in bread.
It has also been used to simulate
in vitro
gastrointestinal digestion of pea or whey protein isolates.


Pepsin is a peptidase used to digest proteins and is commonly used in the preparation of Fab fragments from antibodies. Pepsin, from porcine gastric mucosa, has been used to hydrolyze dry cervical samples in mice. Product P6887 is provided as a lyophilized powder and has been used to digest protein during dietary fiber analysis
.

Biochem/physiol Actions

Preferential cleavage: hydrophobic and aromatic residues in P1 and P1′ postitions. Cleaves Phe-Val, Gln-His, Glu-Ala, Ala-Leu, Leu-Tyr, Tyr-Leu, Gly-Phe, Phe-Phe and Phe-Tyr bonds in the β chain of insulin

Unlike many other peptidases, pepsin hydrolyzes only peptide bonds, not amide or ester linkages. The cleavage specificity includes peptides with an aromatic acid on either side of the peptide bond, especially if the other residue is also an aromatic or a dicarboxylic amino acid. Increased susceptibility to hydrolysis occurs if there is a sulfur-containing amino acid close to the peptide bond, which has an aromatic amino acid. Pepsin will also preferentially cleave at the carboxyl side of phenylalanine and leucine, and to a lesser extent at the carboxyl side of glutamic acid residues. It does not cleave at valine, alanine, or glycine linkages.
Z-L-tyrosyl-L-phenylalani
ne, Z-L-glutamyl-L-tyrosine, or Z-L-methionyl-L-tyrosine may be used as substrates for pepsin digestion.
Pepsin is inhibited by several phenylalanine-containing peptides.


Other Notes

View more information on pepsin at www.
Sigma-Aldrich
.com/enz
ymeexplorer.

Packaging

1, 5, 10 g in poly bottle

250 mg in poly bottle

Unit Definition

One unit will produce a ΔA
280
of 0.001 per min at pH?2.0 at 37°C, measured as TCA-soluble products using hemoglobin as substrate. (Final volume = 16?ml. Light path = 1?cm.)
Safety:

Symbol?


??GHS07, GHS08



Signal word?


Danger




Hazard statements?

H315-H319-H334-H335



Precautionary statements?

P261-P305 + P351 + P338-P342 + P311



Personal Protective Equipment?

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



RIDADR?

NONH for all modes of transport



WGK Germany?

1



RTECS?

SC6132000