商品编号


EXO-FBS-50A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分类


exosome isolation





商品信息

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Overview:
Avoid inadvertently studying bovine exosomes

Fetal bovine serum, or FBS, is an important component of many types of cell culture media. But for researchers interested in isolating exosomes from cultured cells, standard FBS can introduce unwanted complications—bovine exosomes, which can cause significant background issues or interfere with functional studies. Which is why
SBI
developed Exo-FBS, our patented exosome-depleted FBS.



Exosome-sized vesicles removed

Very low levels of CD63-positive cow exosomes

Undetectable levels of cow miRNAs

Comparable growth rates as standard FBS

Interchangeable with standard FBS (add 10% in DMEM or RPMI)


Supporting Data:

High quality and great performance

Exo-FBS has greatly reduced levels of bovine exosomes (Figures 1 and 2), bovine miRNAs (Figure 3), and is even cleaner than ultracentrifuged FBS (Figure 4). Cell growth in media supplemented with Exo-FBS is similar to cell growth in media supplemented with standard FBS (Figure 5).

Exo-FBS has greatly reduced levels of bovine exosomes


Figure 1. NanoSight particle analysis shows low levels of exosomes in Exo-FBS.
While standard FBS contains exosome-sized particles (top panels), Exo-FBS shows almost no particles (bottom panels). Standard FBS and Exo-FBS samples were diluted 1:1000 and then analyzed for particle size and abundance using a NanoSight LM10 instrument.


Figure 2. Bovine α-CD63
ELISA
shows low levels of exosomes in Exo-FBS
CD63 is an exosome-specific
Marker
. An α-CD63
ELISA
of standard FBS and Exo-FBS shows very low levels of CD63 in Exo-FBS, supporting the NanoSight particle analysis which showed low numbers of exosome-sized particles in Exo-FBS (Figure 1). Equal volumes (50 ?l) of either standard FBS or Exo-FBS depleted media supplement were used and the graphed results normalized to the signal level of standard FBS.


Figure 3. qPCR assays show undetectable levels of bovine exosomal miRNAs in Exo-FBS.
While standard FBS contains amplifiable miRNAs (12 of the 72 individual miRNAs tested, left panels), Exo-FBS shows no amplifiable miRNAs (right panels). Standard FBS and Exo-FBS media supplements (4 ml) were treated with Trizol extraction methods to recover exosomal RNAs. RNA was converted to
CDN
A and 72 individual bovine microRNAs were measured by qPCR using
SBI
’s QuantiMir system.


Figure 4. Cells grown in 10% Exo-FBS show comparable growth rates to 10% standard FBS.
HT1080 fibrosarcoma cells, PC-3 prostate cancer cells, MCF-7 breast cancer cells, and HEK293 cells were seeded at either 10,000 or 20,000 cells and then cultured under standard conditions at 37°C with 5% CO2 for 5 days.


Citations:
Rody, WJ, et al. (2017) The use of cell culture platforms to identify novel
Marker
s of bone and dentin resorption.
Orthod Craniofac Res
. 2017 Jun 1;:89-94. PM ID: 28643914
Beltrami, C, et al. (2017) Human Pericardial Fluid Contains Exosomes Enriched with Cardiovascular-Expressed MicroRNAs and Promotes Therapeutic Angiogenesis.
Mol. Ther.
. 2017 Mar 1; 25(3):679-693. PM ID: 28159509
Sun, L, et al. (2017) Exosomes derived from human umbilical cord mesenchymal stem cells protect against cisplatin-induced ovarian granulosa cell stress and apoptosis in vitro.
Sci Rep
. 2017 May 31; 7(1):2552. PM ID: 28566720
Zhou, X, et al. (2017) Exosome production and its regulation of EGFR during wound healing in renal tubular cells.
Am. J. Physiol. Renal Physiol.
. 2017 Mar 29;:ajprenal.00078.2017. PM ID: 28356285
Josson, S, Gururajan, M & WKChung, L. (2017) miRNA Characterization from the Extracellular Vesicles.
bio-protocol
. ; 7(4). Link: bio-protocol
McNicholas, K & Michael, MZ. (2017) Immuno-characterization of Exosomes Using Nanoparticle Tracking Analysis.
Methods Mol. Biol.
. 2017 Dec 12; 1545:35-42. PM ID: 27943205
Nakamura, K, et al. (2017) Exosomes Promote Ovarian Cancer Cell Invasion through Transfer of CD44 to Peritoneal Mesothelial Cells.
Mol. Cancer Res.
. 2017 Jan 1; 15(1):78-92. PM ID: 27758876
Banton, S. (2017) Human peripheral reticulocyte isolation and exosome release in vitro.
Thesis
. ;. Link: Thesis
Oh, HJ, et al. (2017) Convective exosome-tracing microfluidics for analysis of cell-non-autonomous neurogenesis.
Biomat
erials
. 2017 Jan 1; 112:82-94. PM ID: 27750100
Devhare, PB, et al. (2017) Exosome-Mediated Intercellular Communication between Hepatitis C Virus-Infected Hepatocytes and Hepatic Stellate Cells..
J. Virol.
. 2017 Mar 15; 91(6). PM ID: 28077652
Huang, JH, et al. (2017) Systemic Admi
NIST
ration of Exosomes Released from Mesenchymal Stromal Cells Attenuates Apoptosis, Inflammation and Promotes Angiogenesis after Contusion Spinal Cord Injury in Rats.
J. Neurotrauma
. 2017 Jun 30;. PM ID: 28665182
Harischandra, DS, et al. (2017) Environmental neurotoxicant manganese regulates exosome-mediated extracellular miRNAs in cell culture model of Parkinson’s disease: Relevance to α-synuclein misfolding in metal neurotoxicity.
Neurotoxicology
. 2017 Apr 24;. PM ID: 28450057
Crookenden, MA, et al. (2017) Effect of circulating exosomes from transition cows on M
ADI
n-Darby bovine kidney cell function.
J. Dairy Sci.
. 2017 Jul 1; 100(7):5687-5700. PM ID: 28456398
Philley, JV, et al. (2017) Exosome secretome and mediated signaling in breast cancer patients with nontuberculous mycobacterial disease.
Oncotarget
. 2017 Mar 14; 8(11):18070-18081. PM ID: 28160560
Rashed, MH, et al. (2017) Exosomal miR-940 maintains SRC-mediated oncogenic activity in cancer cells: a poss
IBL
e role for exosomal disposal of tumor suppressor miRNAs.
Oncotarget
. 2017 Mar 21; 8(12):20145-20164. PM ID: 28423620
Huang, MB, et al. (2017) Secretion modification region-derived peptide blocks exosome release and mediates cell cycle arrest in breast cancer cells.
Oncotarget
. 2017 Feb 14; 8(7):11302-11315. PM ID: 28076321
Lambrecht, J, et al. (2017) Circulating ECV-Associated miRNAs as Potential Clinical Bio
Marker
s in Early Stage HBV and HCV Induced Liver Fibrosis.
Front Pharmacol
. 2017 Feb 24; 8:56. PM ID: 28232800
Xin, H, et al. (2017) Secondary Release of Exosomes From Astrocytes Contributes to the Increase in Neural Plasticity and Improvement of Functional Recovery After Stroke in Rats Treated With Exosomes Harvested From MicroRNA 133b-Overexpressing Multipotent Mesenchymal Stromal Cells.
Cell Transplant
. 2017 Feb 16; 26(2):243-257. PM ID: 27677799
Ye, W, et al. (2017) Plasma-derived exosomes contribute to inflammation via the TLR9-NF-κB pathway in chronic heart failure patients.
Mol. Immunol.
. 2017 Jul 1; 87:114-121. PM ID: 28433888
Hosseinkhani, B, et al. (2017) Direct detection of nano-scale extracellular vesicles derived from inflammation-triggered endothelial cells using surface plasmon resonance.
Nanomedicine
. 2017 Jul 1; 13(5):1663-1671. PM ID: 28366819