商品编号


EXFT10A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分类


exosome engineering


商品信息

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Overview:
Putting exosomes to work: Load your cargo directly into isolated exosomes

With Exo-Fect, you can turn isolated exosomes into cargo delivery vehicles that introduce RNAs, DNAs (including plasmids), and even small molecules into recipient cells. Simply combine isolated exosomes with Exo-Fect and your desired cargo, and in less than an hour your loaded exosomes will be ready to add to recipient cells.




Easy-to-use with a fast and straightforward lo
ADI
ng protocol

Introduces a wide range of biomolecules directly into isolated exosomes:

RNAs, including siRNAs, miRNAs, and mRNAs

DNAs, including plasmids

Metabolites and other small molecules



Enables non-viral transduction and stable cell line creation

Provides an alternative gene delivery method for hard-to-transfect cells


All Exo-Fect Kits come with a Texas Red-labeled positive control, non-targeting siRNA to confirm exosome transfections. Pair with our EV-Entry Reagent and maximize cargo uptake by recipient cells.

How It Works:
Quickly and easily load cargo into isolated exosomes


Simply combine the isolated exosomes with Exo-Fect Reagent and cargo, perform two incubations for a total of 40 minutes, and then pellet your exosomes. The Kit comes with all of the reagents you need to load cargo into exosomes and concentrate them for delivery to target cells. The protocol takes less than an hour and is highly efficient at lo
ADI
ng cargo into exosomes for transport and delivery.

Exo-Fect is also compat
IBL
e with the EV-Entry Reagent, which maximizes cargo uptake by recipient cells.
Citations:
Torri, A, et al. (2017) Extracellular MicroRNA Signature of Human Helper T Cell Subsets in Health and Autoimmunity.
J. Biol. Chem.
. 2017 Feb 17; 292(7):2903-2915. PM ID: 28077577
Torri, A, et al. (2017) JBC Papers in Press. Published on January 11, 2017 as Manuscript M116. 769893.
Journal of
BIOLOG
ical chemistry
. 2017 Jan 11;. Link: Journal of
BIOLOG
ical chemistry
Zhu, Z, et al. (2017) Macrophage-derived apoptotic bodies promote the proliferation of the recipient cells via shuttling microRNA-221/222.
J. Leukoc. Biol.
. 2017 Mar 8;. PM ID: 28274991
Downs, CA, et al. (2017) Hydrogen peroxide stimulates exosomal cathepsin B regulation of the receptor for advanced glycation end-products (RAGE).
J. Cell. Biochem.
. 2017 Jun 15;. PM ID: 28618037
Srivastava, A, et al. (2016) Exploitation of Exosomes as Nanocarriers for Gene-, Chemo-, and Immune-Therapy of Cancer.
J Biomed Nanotechnol
. 2016 Jun 1; 12(6):1159-73. PM ID: 27319211
Jella, KK, et al. (2016) Exosomal GAPDH from Proximal Tubule Cells Regulate ENaC Activity.
PLoS ONE
. 2016 Nov 1; 11(11):e0165763. PM ID: 27802315