商品编号


EXOLP5A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分类


exosome isolation




商品信息

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Overview:
Efficiently pre-clear serum or plasma lipoproteins before exosome isolation

For researchers who specifically want to study exosomal RNA or proteins, removing non-exosomal molecules prior to treatment with ExoQuick
?
can lead to data that more reliably reports on exosomal RNA or proteins. Because both high density and low density lipoproteins (HDLs and LDLs) can contain miRNAs
1
, lipoprotein removal is recommended prior to exosome isolation from serum or plasma. ExoQuick-LP
?
is specifically designed to remove normally-occurring lipoprotein particles from serum or plasma prior to ExoQuick precipitation of exosomes.


The ExoQuick-LP Kit contains ExoQuick and proprietary pre-clearing reagents that efficiently deplete lipoprotein particles from plasma or serum prior to ExoQuick treatment.

Choose the right ExoQuick for your biofluid:




Catalog #

Product

Biofluid





EXOQ5A-1, EXOQ20A-1

The Original ExoQuick

For serum, plasma, and ascites fluid



EXOTC10A-1, EXOTC50A-1

ExoQuick-TC
?

For all other biofluids



EXOLP5A-1

ExoQuick-LP

For removing contaminating lipoprotein particles from plasma or serum before ExoQuick precipitation



EXOQ5TM-1

ExoQuick Plasma Prep with Thrombin

For de-fibrinating plasma before exosome isolation for efficient recovery and high yields



EXOCG50A-1

ExoQuick-CG

For preparing exosomes used in pre-clinical
in vivo
applications



EQPL10A-1, EQPL10TC

ExoQuick PLUS and ExoQuick-TC PLUS

For sensitive applications such as mass spectrometry, exosome labeling, and
in vivo
/
ex vivo
exosome delivery





REFERENCES


Vickers KC,
et al
. MicroRNAs are transported in plasma and delivered to recipient cells by high-density lipoproteins.
Nat Cell Biol
. 2011 Apr;
13
(4):423-33.


Supporting Data:

See ExoQuick-LP performance data

With ExoQuick-LP, up to 90% depletion of lipoprotein particles from serum or plasma can be achieved.
SBI
’s exosome isolation products help you take full advantage of the power of exosomes.


ExoQuick-LP efficiently clears lipoproteins from serum

ExoQuick-LP treatment of serum results in lower amounts of lipoprotein
Marker
s (ApoA-1 for HDLs, and ApoE for LDLs) that co-isolate with exosomes than treatment of serum with ExoQuick alone, as shown by
ELISA
(Figure 1).


Figure 1. ExoQuick-LP efficiently clears lipoproteins from serum.

To test for exosome recovery after ExoQuick-LP pre-clearance, we used
SBI
’s Exosome
ELISA
kits (Cat.# EXOEL-CD81A-1, EXOEL-CD63A-1, and EXOEL-CD9A-1) to measure the approximate number of exosomal particles present in ExoQuick serum pellets with or without treatment with ExoQuick-LP (Figure 2). While there were some differences in observed particle numbers in untreated vs treated samples, overall yields were quite similar, suggesting minimal loss of material using ExoQuick-LP.

Figure 2. ExoQuick-LP delivers robust exosome yields.

Serum exosomes pre-treated with ExoQuick-LP were also tested for size and concentration using NanoSight tracking analysis with a 1:100 dilution of isolated exosomes (Figure 3). We observed that there are minimal differences in particle sizes using ExoQuick-LP vs untreated controls, suggesting that exosomes are intact and structurally similar to exosomes isolated using standard ExoQuick isolation methods.

Figure 3. ExoQuick-LP delivers particles of the size expected for exosomes.