商品编号


EG-1021



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Genome Engineering




1,000 Units

商品信息



Description


Recombinant Cre recombinase (TAT-Cre) was purified from an HEK293 cell line expressing enhanced form of Cre Recombinase from bacteriophage P1. This Cre recombinase has an N-terminal 6XHis tag, a Tat peptide (GRKKRRQRRRPPAGTSVSL) and an NLS sequence (PKKKRKV). Tat-Cre is the most effective protein in transduction (in vivo) and subsequent recombination compared to other forms of Cre recombinases, e.g., HNC, TCH6, HC, HNCM, CH.





Applications






In vitro LoxP recombination for subcloning or vector/clone engineering



Transduction into cultured cells including stem cells
ex vivo







Source

HEK 293 cells



Activity

A standard of 100 Units is defined as the amount of Tat-Cre in 1.0 mL of tissue culture medium that is required to induce 90% GFP expression in a HEK 293T loxP reporter cell line assay.



Properties


QC
: HEK293T- Cre reporter cell line 80~100% recombination efficiencies.


Figure 1
. QC assay of Tat-Cre. Adding? 50 and 100 units of Tat-Cre to HEK 293T cells transfected with LoxP-RFP-Stop-LoxP-GFP reporter plasmid DNA.

Endotoxin Levels
: < 0.1 EU/ug




Storage

-20?C ~ -80?C



Shipping

dry ice