商品编号


EG-1020



品牌


Excellgen



公司


Excellgen, Inc.



公司分类


Genome Engineering




2000 units

商品信息



Description


Cre Recombinase exosome-like vesicles are 20 to 50 nm lipid vesicles isolated from cultured mammalian cells. These vesicles encapsulate high concentration of NLS-Cre recombinase, but do not contain virus and nucleic acids (plasmid DNA, RNA etc). Addition of 10 ~ 50 ?l of the vesicles to reporter cells (
such as
HEK293, Hela, NIH 3T3) and primary cells results in
loxP
recombination. The transduction efficiency ranges from 60 to 99%.





Applications






In vitro LoxP recombination for subcloning or vector/clone engineering



Transduction into cultured cells including stem cells for
ex vivo
LoxP recombination







Size

2000 units



Activity

A standard of 100 Units is defined as the amount of HTN-Cre in 1.0 mL of tissue culture medium that is required to induce 90% RFP expression in a HEK293T loxp reporter cell line assay



Endotoxin

< 0.1 EU/ug



Storage

4?C ~ -80?C



Shipping

dry ice