商品编号


CC02810



品牌


Lucigen



公司


Lucigen



公司分类


General Cloning & Library Construction



Size

10 x 50 ?l

商品信息

General cloning strain

Clone large inserts and transform large plasmids - up to at least 145 kb plasmid DNA.

Achieve high efficiencies with convenient chemically competent format: >5 × 10
8
cfu/?g pUC19 DNA.

Ideal for cloning, subcloning and plasmid preparation due to endA and recA mutations.





Applications

Routine cloning of DNA up to 200 kb.

The highly versatile TransforMax? EC100?
E. coli
competent cells are ideal for most cloning applications. The cells provide very high transformation efficiency when tested against a wide range of supercoiled DNAs as well as DNA directly from a ligation reaction (Table 1).

Benefits

High transformation efficiency with clones of all sizes, including BAC clones (Table?1).

lacZΔM15
for blue/white screening of recombinants.

Restriction minus [
mcrA, Δ(mrr-hsdRMS-mcrBC)
] enables efficient cloning of methylated DNA.

Endonuclease minus (
endA1
) to ensure high yields of DNA.

Recombination minus (
recA1
) for greater st
ABI
lity of large cloned inserts.



DNA

TransforMax? EC100? Chemically Competent
E.?coli



TransforMax? EC100? Electrocompetent
E.?coli


pUC19

1.4 × 10
8

1.4 × 10
10

8.1-kb Clone

1.3 × 10
7

Not tested

13.1-kb Clone

4.3 × 10
6

1.3 × 10
9

23.1-kb Clone

9.2 × 10
5

3.0 × 10
8

145-kb BAC Clone

Not tested

7 × 10
7

13.1-kb clone directly from a ligation reaction

2.2 × 10
5

2.1 × 10
7


Table 1. Comparison of the transformation efficiencies of TransforMax? EC100?
E.?coli
with a variety of DNAs.
Transformations were performed using 50 ?l of competent cells and either supercoiled DNAs of the indicated sizes or a 1-?l aliquot from a standard 10-?l ligation reaction. Results shown are in cfu/?g of DNA and are the average transformation efficiencies obtained from several trials.



Genotype

F
-
mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ
-
rpsL (Str
R
) nupG

TransforMax EC100 Electrocompetent
E. coli


Transformation efficiency of >1 × 10
10
cfu/?g of pUC19.

TransforMax EC100 Chemically Competent
E. coli


Transformation efficiency of >5 × 10
8
cfu/?g of pUC19.


ORDER INFORMATION
Includes pUC19 control DNA.