商品编号


60081-1



品牌


Lucigen



公司


Lucigen



公司分类


General Cloning & Library Construction



Size

12 rxns (SOLOs)




商品信息


Direct replacements for standard cloning strains (e.g., DH5α?, DH10B?, JM109, TOP10, XL1-Blue, etc.)

Optimized genetics for high yields: ?phage T1 resistant, endonuclease and recombination minus, blue/white screening-capable.

Available in a range of high transformation efficiencies (5 × 10
9
to 4 × 10
10
cfu/?g).

Convenient packaging options.


Looking for chemically competent version?

Free Sample Available

?





Lucigen
’s
E. cloni
competent cells share the most useful genetic elements of standard cloning strains like DH5α?, DH10B?, JM109, TOP10, etc. and directly replace them in cloning protocols. However,
E. cloni
electrocompetent cells incorporate a unique manufacturing technology that increases transformation efficiency, recombinant yields and reli
ABI
lity (Figures 1 and 2), while decreasing costs. ?These cells provide solutions for a wide range of applications at economical prices.

Choice of strain:



E. cloni
10G Competent Cells.
Library construction, cloning, subcloning, and plasmid isolation with or without blue/white screening.


E. cloni
10GF′Competent Cells.
Contain the F' plasmid for infection with M13 to produce ssDNA.


Choice of efficiency:



E. cloni
10G SUPREME Electrocompetent Cells

>
4 × 10
10
cfu/?g pUC DNA
SUPREME Cells have the highest transformation efficiency available from any supplier. Choose SUPREME Cells for the most demanding cloning situations, such as construction of large, high complexity libraries or cloning difficult targets, which require the greatest number of transformants poss
IBL
e.


E. cloni
10G & 10GF? ELITE Electrocompetent Cells
>
2
×
10
10
cfu/?g pUC DNA
ELITE Cells have twice the transformation efficiency compared to “ultra high efficiency” cells from other suppliers. ELITE Cells provide large numbers of transformants from hard-to-clone fragments or limited DNA at a lower price.


E. cloni
10G CLASSIC Electrocompetent Cells

>
5
×
10
9
cfu/?g pUC DNA
CLASSIC Cells are high efficiency cells with a substantially lower cost per reaction. These cells are the most economical choice for standard cloning and library construction. 10G CLASSIC Cells are available in larger package sizes for convenient use in higher volume cloning applications.



Custom Competent Cells

Bulk
Lucigen
cells or your strain of
E. coli
, made to the highest poss
IBL
e transfection efficiency with custom dispensing! See
Custom Competent Cells page
for detailed information.



Plasmid cloning.
E. cloni
10G SUPREME Cells provide recombinant yields higher than the highest efficiency cells offered by a le
ADI
ng supplier (Figures 1 and 2)








?










Figure 1.

E. cloni
10G SUPREME Electrocompetent Cells consistently outperformed “ultra high efficiency” cells from a le
ADI
ng supplier. Both strains were transformed with 10 pg of pUC19 (n=16).



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Figure 2.
Transformation efficiency comparison of
E. cloni
10G SUPREME Cells (
Lucigen
) and Mega DH10B cells (
Invitrogen
). Competent cells were transformed according to the manufacturer’s instructions with the same pUC19 DNA control (provided with the
Invitrogen
cells)






Table 1.
E. cloni
Competent Cells for Cloning and Library Construction







E. cloni
Cell Lines



Transformation Efficiency
(cfu/?g pUC DNA)




Cloning Methylated DNA




BAC, Cosmid Cloning




Blue/White Screening





10G SUPREME Electrocompetent




>
4 × 10
10



YES



YES



YES
without IPTG induction





10G ELITE Electrocompetent




>
2 × 10
10



YES



NO



YES
without IPTG induction





10G CLASSIC Electrocompetent




>
5 × 10
9



YES



NO



YES
without IPTG induction





10GF? ELITE Electrocompetent




>
2 × 10
10



YES



NO



YES
IPTG induction required






Efficiencies


E. cloni
?10G SUPREME Electrocompetent Cells
?
>
?4 × 10
10
?cfu/?g pUC DNA

E. cloni?
10G & 10GF? ELITE Electrocompetent Cells?
>
?2?×?10
10
?cfu/?g pUC DNA

E. cloni?
10G CLASSIC Electrocompetent Cells
?
>
?5?×?10
9
?cfu/?g pUC DNA?




ORDER INFORMATION
Competent Cells include Control DNA and Recovery Medium, and are packaged as
SOLOs
(1 transformation per tube),
DUOs
(2 transformations per tube), or
SixPacks
(6 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE:
Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all
Lucigen
Competent Cells. Please contact
Lucigen
.