商品编号


60602-1



品牌


Lucigen



公司


Lucigen



公司分类


General Cloning & Library Construction



Size

12 rxns (DUOs)

商品信息


Direct replacements for standard cloning strains (e.g., DH5α?, DH10B?, JM109, TOP10, XL1-Blue, etc.).

Optimized genetics for high yields: endonuclease and recombination minus, for blue/white screening-capable.





Lucigen
’s
E. cloni
competent cells share the most useful genetic elements of standard cloning strains like DH5α? and directly replace them in cloning protocols. ?
E. cloni
chemically competent cells provide the performance researchers need with ease of use—only common laboratory equipment is required. ?These cells provide solutions for a wide range of applications at economical prices.


E. cloni
5-alpha Chemically Competent Cells
>
1 × 10
8
cfu/?g DNA
Useful for routine cloning, subcloning, and plasmid isolation with or without blue/white screening.


Custom Competent Cells


Bulk
Lucigen
cells or your strain of
E. coli
, made to the highest poss
IBL
e transfection efficiency with custom dispensing!
See
Custom Competent Cells page
for detailed information.


Table 1.
E. cloni
Competent Cells for Cloning






E. cloni
Cell Lines



Transformation Efficiency
(cfu/?g pUC DNA)




Cloning Methylated DNA




BAC, Cosmid Cloning




Blue/White Screening





5-alpha Chemically Competent




>
1 × 10
8
cfu/?g DNA



NO



NO



YES
No IPTG induction required





?



ORDER INFORMATION
Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE:
Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all
Lucigen
Competent Cells. Please contact
Lucigen
.