商品编号


60522-1



品牌


Lucigen



公司


Lucigen



公司分类


Phage Display Library Applications



Size

12 rxns (DUOs)


商品信息


Perfect for antibody phage display or peptide phage display library creation

The highest efficiency TG1 competent cells available: ≥4 × 10
10
cfu/?g.

Your only source for electrocompetent SS320 and ER2738 cells

Amber suppressor and non-amber suppressor lines for maximum flexibility

High efficiency
MC1061 F- cells
for cloning or control reactions

Create larger libraries; speed discovery

Bulk custom dispensing available with all of our phage display cells


Free Sample Available

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MC1061 F- Electrocompetent Cells.
A non-amber suppressor strain, identical to SS320 except that it lacks the F′episome. Highly efficient (≥4 × 10
10
cfu/?g) electrocompetent cells for general cloning or Phage Display. Note that as an F- strain, it cannot be used for re-infection by filamentous phage.?
This strain not included in the sample kit.




TG1 Electrocompetent Cells.
An amber suppressor strain (
sup
E) prepared as highly efficient (≥4 × 10
10
cfu/?g) electrocompetent cells for phage display library screening. These cells are used for phage display and protein expression.



SS320 Electrocompetent Cells.
A non-amber suppressor strain (sometimes called MC1061F') prepared as highly efficient (≥4 × 10
10
cfu/?g) electrocompetent cells for phage display library screening. These cells have the highest available transformation efficiency of any strain used for phage display.


ER2738 Electrocompetent Cells.
An amber suppressor strain (
gln
V) prepared as highly efficient (≥2 × 10
10
cfu/?g) electrocompetent cells for phage display library screening. This strain is recommended for use with New England Biolab's Ph.D.? Phage Display Kits.










Figure 1.
Transformation efficiency of
Lucigen
's electrocompetent bacterial cells for phage display compared to competitor's specification.






Table 1. Competent Cells for Phage Display libraries






Cell Lines



Transformation Efficiency
(cfu/?g pUC DNA)




Cloning Methylated DNA




BAC, Cosmid Cloning




Blue/White Screening






TG1 Electrocompetent




>
4 × 10
10



YES



NO



YES
IPTG induction required





SS320 (MC1061 F') Electrocompetent




>
4× 10
10



YES



NO



YES
IPTG induction required





MC1061 F- Electrocompetent




>
4× 10
10



YES



NO



YES
IPTG induction required





ER2738 Electrocompetent




>
2 × 10
10



YES



NO



YES
IPTG induction required







Custom Competent Cells

Bulk
Lucigen
cells or your strain of
E. coli
, made to the highest poss
IBL
e transfection efficiency with custom dispensing!
See Custom Competent Cells for detailed information.



ORDER INFORMATION
Competent Cells include Control DNA and Recovery Medium, and are packaged as
DUOs
(2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.

PLEASE NOTE:
Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all
Lucigen
Competent Cells. Please contact
Lucigen
.