商品编号


60240-2



品牌


Lucigen



公司


Lucigen



公司分类


CRISPR, Large or Difficult Fragment Cloning




24 rxns (DUO)



商品信息

Clone repetitive sequences and lentiviral libraries


St
ABI
lize direct repeats and create lentiviral constructs

Generate lentiviral guide RNA libraries

Recommended in CRISPR GeCKO library protocols

Choose electrocompetent or chemically competent cells

Highest efficiency commercially available cells for lentiviral cloning:? over 1 × 10
7
cfu/?g (chem) or 1 × 10
10
cfu/?g (electro)


Transformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra Recovery Medium.
? Order extra Recovery Medium below.

Free Sample Available




Conserve clones that contain unstable sequences!


Maintain unstable DNA.

Lucigen
’s Endura Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains. Clones with inverted repeats or other sequences prone to recombination are commonly found in retroviral genes, and require cells such as Endura to be propagated stably.


Excellent value and efficiency!
Switch to Endura cells today and experience a higher level of efficiency and a savings in your cloning budget.


Chemical or Electrocompetent.
Whichever method of transformation you prefer,
Lucigen
can give you better efficiency and/or better prices.





Figure 1.
Cost per reaction of chemically competent cells of clone-st
ABI
lizing strains from other suppliers.





Figure 2.
Transformation efficiencies of electrocompetent clone-st
ABI
lizing strains.



ORDER INFORMATION
Endura? Competent Cells include Control DNA and Recovery Medium, and are packaged as?
SOLOs
?(1 transformation per tube) or
DUOs
?(2 transformations per tube) as indicated. Endura Electrocompetent cells are provided in DUOs format only. Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.


PLEASE NOTE:
?Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all
Lucigen
Competent Cells.?Please contact
Lucigen
.

NOTE:? Transformation of Endura Electrocompetent Cells with GeCKO lentiviral libraries may require extra Recovery Medium.
? The Zhang lab protocol (available from Addgene) recommends ~2 mL of Recovery Medium per transformation. ?
Lucigen
Endura kits provide 1 mL of Recovery Medium per transformation. ?Order extra Recovery Medium above or visit the Recovery Medium product page here!