商品编号


60224-2



品牌


Lucigen



公司


Lucigen



公司分类


CRISPR, Large or Difficult Fragment Cloning




12 rxns (SOLOs)

商品信息


Cells required for transformation and propagation of pJAZZ? Vectors.

Highest available transformation efficiencies for library construction and screening.

Optimized for high yields of DNA.




BigEasy?-TSA? Electrocompetent Cells (
>
2 × 10
10
cfu/?g pKanR DNA) are for use with
Lucigen
’s pJAZZ?-OC or OK Linear Vectors in the BigEasy v2.0 Linear Cloning System. The TSA Cells have the
tel
N gene required for high efficiency transformation, and the
ant
A gene required for copy number control with the pJAZZ vector, integrated into the host genome. These cells also give high yield and high quality plasmid DNA due to the
end
A1 and
rec
A1 mutations. They contain the
mcr
and
mrr
mutations, allowing methylated genomic DNA that has been isolated directly from mammalian or plant cells to be cloned without deletions or rearrangements.


Table 1.
E. cloni
Competent Cells for BAC, Large or Difficult Fragment Cloning






E. cloni
Cell Lines



Transformation Efficiency
(cfu/?g pUC DNA)




Cloning Methylated DNA




BAC, Cosmid Cloning




Blue/White Screening





BigEasy-TSA Electrocompetent




>
2 × 10
10



YES



No



YES
IPTG induction required






BigEasy-TSA
: F-
mcr
A Δ(
mrr-hsd
RMS-
mcr
BC) Φ80d
lac
ZΔM15 Δ
lac
X74
end
A1
rec
A1
ara
D139 Δ(
ara,leu
)7697
gal
U
gal
K
rps
L
nup
G &lam
BD
a;-
ton
A
Amp
R

sopAB tel
N
ant
A



ORDER INFORMATION
Competent Cells include Control DNA and Recovery Medium, and are packaged as SOLOs (1 transformation per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise. PLEASE NOTE: Bulk quantities (10 times larger than the largest retail package size below) and/or custom packaging are available at very attractive prices for all
Lucigen
Competent Cells. Please contact
Lucigen
.