商品编号


60701-1



品牌


Lucigen



公司


Lucigen



公司分类


CRISPR, Large or Difficult Fragment Cloning




12 rxns

商品信息

High efficiency cells to create uracil-containing DNA for site-directed mutagenesis


Sole source of highly efficient Electrocompetent cells (1 × 10
9
cfu/?g)

ung- and dut- mutations to generate DNA with some uracil residues

Ideal genotype and efficiency for Kunkel directed mutagenesis method
1
.


1
Kunkel T.A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection.?
Proc. Natl. Acad. Sci.

52:
488-492.




Lucigen
is the sole provider of high-efficiency electrocompetent CJ236 cells. This cell line was created by adding the pCJ105 plasmid to BW313 cells
2
, and is ideally suited to the Kunkel directed mutagenesis method. This method relies on the cells producing plasmid DNA containing some uracil residues. The dut- and ung- mutations prevent the cells from removing uracil from DNA caused by misincorporation or dCTP deamination. Note that these cells contain an F’ which confers resistance to Chloramphenicol at low levels.

As Electrocompetent cells, the transformation efficiency is 1 × 10
9
cfu/?g.

Functional genotype:
[F’ Tra+ Pil+ (CamR)]
ung-1 relA1 dut-1 thi-1 spoT1 mcrA

2
Joyce, C.M., N. Grindley 1984. Method for determining whether a gene of
Escherichia coli
is essential: application to the polA gene.
J.Bacteriol.
158:
636-643

ORDER INFORMATION
Competent Cells include Control DNA and Recovery Medium, and are packaged as DUOs (2 transformations per tube). Recovery Medium is also available separately. The specified transformation efficiencies are with pUC DNA, unless indicated otherwise.