商品编号


ECP09500



品牌


Lucigen



公司


Lucigen



公司分类


TransforMax? EC100D?pir+andpir-116 ElectrocompetentE. coli



Size

5 x 100 ?l

商品信息

Rescue cloning of EZ-Tn5? transposed elements


Rescue transposition mutants created using the EZ-Tn5? R6K?
ori
Kan-2> Insertion Kit
or Tnp Transposome? Kit


Propagate plasmids tagged with the EZ-Tn5? R6K?
ori
Kan-2> Insertion Kit





The TransforMax? EC100D?
pir
+
Electrocompetent
E. coli
and TransforMax? EC100D?
pir
-116 Electrocompetent
E. coli
each constitutively express the π protein (the
pir
gene product) for replication of plasmids containing the R6Kγ origin of replication (R6Kγ
ori
). The cells are derived from Epicentre's TransforMax? EC100? Electrocompetent
E. coli
by P1 phage transduction with a strain containing the
pir
+
or
pir
-116 gene linked to a dihydrofolate reductase (DHFR)
Marker
.

The TransforMax EC100D
pir
+
cells will maintain R6Kγ
ori
containing plasmids at approximately 15 copies per cell,
1
for cloning of potentially toxic or unstable DNA sequences. The TransforMax EC100D pir-116 cells are for high-copy propagation of up to 250 rescue plasmid copies per cell.
1

Benefits

Accepts large clones for unbiased propagation and st
ABI
lity of large rescue clones.

lacZΔM15
for blue/white screening of recombinants.

Restriction minus
[mcrA, Δ(mrr-hsdRMS-mcrBC)
] enables efficient cloning of methylated DNA.

Endonuclease minus (
end
A1) to ensure high yields of DNA.

Recombination minus (
rec
A1) for greater st
ABI
lity of large cloned inserts.

Genotypes


TransforMax EC100D
pir
+
Electrocompetent
E. coli
:
F
-
mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (Str
R
) nupG pir
+
(DHFR)

TransforMax EC100D
pir
-116 Electrocompetent
E. coli
:
F
-
mcrA Δ(mrr-hsdRMS-mcrBC) φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL (Str
R
) nupG pir-116(DHFR)


Transformation Efficiency
Greater than 5 × 10
9
colony forming units (cfu)/?g with pR6Kan control DNA.

References

Metcalf, W.W.
et al.
(1994)
Gene

138
, 1.


ORDER INFORMATION
Each product contains 5 tubes each containing 100 µL of electrocompetent cells, which is enough cells for 10 electroporations. A control vector containing and R6Kγori is also included with each product.