商品编号


NB-1001



品牌


Signosis



公司


Signosis



公司分类


miRNA Research




1 Ea

商品信息

Description:
Abundance of mammalian miRNAs varies dramatically in tissues. For example, expression of miR-133 is high in skeletal muscle, moderate in heart, and no detection in other tissues. To detect low expression of miRNAs requires high sensitive methods.
Signosis
’ highly sensitive miRNA northern blot assay is 10 to 100 times more sensitive than conventional biotin-based chemiluminescent detection.
Principle:
RNA samples are separated through gel electrophoresis and transferred onto a membrane. Expression of a specific miRNA is detected with a biotin-labeled probe. This regular Northern blot assay is suitable to most moderate to high expressors of miRNA. High sensitive Northern blot (NB-1001 and HP-XXXX) is needed to analyze low expressors of miRNA. The biotin-labeled probes contain two moieties – complementary sequence of the miRNA and a tag sequence. The tag sequence is then detected by an amplifier enriched with biotin molecules.

Data:

Highly sensitive miRNA northern blot analysis of miR-133 in skeletal muscle total RNA and miR-21 from Hela cell total RNA.