商品编号


GWB-001031



品牌


GenWay



公司


GenWay



公司分类


Molecular Biology Products



Size

10 ?g

商品信息

Description:
pOET2N/C_6xHis is a baculovirus transfer vector designed for high level expression of foreigngenes under the powerful AcMNPV polyhedron (polh) promoter. The vector encodes an Nterminal6×His-Tag? fusion sequence that may be utilized if the insert includes a stop codon.This greatly eases the purification of the recombinant protein since the 6×His-containing fusionproteins bind with high affinity to Ni-NTA Agarose. If required, the 6×His-Tag? can be removedby incubating the fusion protein in the presence of the proteinase cleavage enzyme Thrombin.There is also a 6×His-Tag?for C-terminal fusions where the inserts own start codon can be usedto replace the start codon supplied in pOET2N/C_6xHis. pOET2N/C_6xHis is smaller than otheravailable transfer vectors (4626 bp) which greatly facilitate the cloning steps. It has a Col E1origin of replication and an ampicillin resistance gene for selection in E. coli. The polh sequenceshave been replaced by a multiple cloning site (MCS) containing unique restriction sites forinsertion of the foreign gene in the correct orientation, as shown on the circular map. Thecoding strand of the MCS as transcribed from the polh promoter is shown below the circularmap. The PacI site at the end of the MCS provides translational stop codons in all three re
ADI
ngframes for expression of truncated proteins. The AcMNPV sequences flanking the gene in thetransfer vectors MCS allow recombination with the viral DNA to insert the expression cassetteinto the polh locus. pOET2N/C_6xHis is compat
IBL
e with any baculovirus system that utilizeshomologous recombination in insect cells.
Additional Information:




Name

pOET 2 N/C_6xHis? Transfer Vector



Concentration

Lot Specific



St
ABI
lity

1 year



Storage

-20C



Intended Use

Research Use Only