商品编号


IC-1003



品牌


Gentarget



公司


Gentarget



公司分类


Eco PCR Cloning Kits




1 Ea

商品信息

?

PCR cloning kit to make high-yield E Coli expression vector for expressing N-term His tagged protein. It is specially designed for expressing toxic proteins with?self-controlled low basal level. A Non-T7-based (pEco-BAD-nHis) vector was built-in with the cloning cells, and the expression is induced by L-ar
ABI
nose in a dose-dependent mannal?(please see product manual for vector map and details.). Full vector sequenceing can be download from our website.
?

The clones after sequencing verified, can be directly used for expression without the 2nd transformation into another expression strain.

Kit contents:??


10 tubes x 50ul/ea of?vector built-in, Cloning-Ready cells, for 10 cloning reactions;

1 tube x?5 ul of positive PCR cloning control;

1ml of 2% L-ar
ABI
nose;

Forward and reverse sequencing primers: 15ul/ea at 25ng/ul;


See
Product Manual
(.pdf) for details.

Cat# IC-1003