Gentarget/hsa-miR-125b-5p (MIMAT0000423) miRNA expression lentivirus/miR#178/1 Ea
基因编辑
商品编号
miR#178
品牌
Gentarget
公司
Gentarget
公司分类
microRNA lentivirus
1 Ea
商品信息
Pre-made micro RNA exrepssion lentivirus for human
hsa-miR-125b-5p (
MIMAT0000423
)
:
The ?human microRNA?precursors and its native context sequences (upstream and downstream flanking genomic sequences) was cloned into a
Gentarget
’s miRNA expression lentivector:?
pLenti-TetCMV(GFP-3UTR/miRNA)-
Rsv(Puro)
. The miRNA cloned at 3’UTR or GFP
Marker
under
an optional induc
IBL
e CMV promoter
. ?It can be used for constitutive miR expression or used as induc
IBL
e miR expression when the
TetR represso
r
is prescent. The GFP signal provides a real-time monitor for the miRNA expression levels and the lentivirus transduction efficiency.
The cloned miRNA express precursor sequence is listed below.
Note:
?the Red-highlighted region is the
miR Stem-loop
sequence:?
ACCATACCACCTGTTTGTTGCATCTTAGTTATGAACCTCGAACAGA
AAT
TGCCTGTCATT
CTTGTTTTGCTTTGCTTTGTCTCAAGAAAGAAAACATTGT
TGCGCTCCTCTCAGTCCCTG
AGACCCTAACTTGTGATGTTTACCGTTTAA
ATCC
ACGGGTTAGGCTCTTGGGAGCTGCG
AGTCGTGCT
TTTGC
ATCC
TGGA
AAT
TTGGTGG
AAT
TTTATTCTTTAAAGCAAAAACAAAA
GAAAAGAAAGTTTGTCTGAGGTGATTGAGTATACCTCTGAGGTTTTCAT
It guarantees to generate the matured miRNA sequence as follows:
UCCCUGAGACCCUAACUUGUGA
See?
Produce manual
?for details.
Amounts:
?
1)
500 ul
of?ready-to-use specific miRNA expression lentivirus in DMEM-medium containing 60 ug polybrene (10x).
2)
500 ul
of?ready-to-use?Negative-control miRNA expression lentivirus in DMEM-medium containing 60 ug polybrene (10x). (
Note
: The miR-Control ?does not transcribe any miRNA sequences, but has the same backbone with GFP
Marker
).
Virus titer:
?1×10
7
?IFU/ml,
Shipping:
?overnight shipment in dry-ice package. (
Note: This product is pre-made in stock, ready to ship via overnight dry-ice package
)
CAT#:
miR#178
产品货号:8860.0