商品编号


ULVP-337



品牌


Gentarget



公司


Gentarget



公司分类


Ultra titer lentivirus



Size

1 Ea

商品信息

Pre-made Lentiviral particles?expressing? nuclear permeable
CRE?recombinase
?under an?optional induc
IBL
e?
suCMV?
promoter. A?
GFP
?
Marker
was?co-expressed under the same promoter. CRE?and GFP were?bicistronically expressed?as individual protein mediated by a 2A element. ?

CRE
contains a
NLS (nuclear localization signal)
?at its N-term. This?
nuclear permeant?CRE
?increases the recombination events
in vivo
. A
Blasticidin

Marker
was expressed under RSV promoter. Particles allows to sort or select transduced cells for long term expression via
GFP signal
or via
blasticidin antibiotic
selection.

NLS-CRE and GFP
are? constitutively expressed in high level, and optionally, also can be used as tetracycline induc
IBL
e expression?as desired. GFP signal provides a convenient, realtime monitoring the?particles performance.??

Cre protein, from bacteriophage P1, catalyzes recombination between 34 base pair target sequences,?named
lox sites
. CRE lentiviral particles?can?catalyze?the jointing event
In Vivo
?in the presence of?lox sites. Purified CRE enzyme can joint individual plasmids containing lox sites.

See?
Product Manual
?for details (.pdf).

Particles was concentrated via our proprietary procedures, and re-s
USP
ended in PBS buffer for ultra high titer virus, suitable for all types of?
in vivo
?
applications
.

Amount: ?50 ul/per vial, at 1 x 10e9?IFU/ml in PBS.?

Cat#: ULVP-337